Hypoxia Promotes The Proliferation Of Cervical Carcinoma Cells Through Stimulating The Secretion Of IL-8

OBJECTIVE:To explore whether hypoxia and interleukin8(IL-8) regulate the viability and apoptosis of cervical carcinomas cells and the possible mechanism.METHODS: We evaluated the expression of hypoxia inducible factor-la (HIF-1α), IL-8and its receptors (CXCRl and CXCR2) in cervical cancer and cervicitis tissues by immunhistochemistry. Then the effects of hypoxia and IL-8on the viability and apoptosis of HeLa and SiHa cells were detected by the SRB and apoptosis assays.RESULTS:Here we observed that the expression of HIF-1α, IL-8and CXCRl in cervical cancer tissues was significantly higher than that in cervicitis tissues. Hypoxic condition stimulated the secretion of IL-8and the expression of CXCRl and CXCR2on HeLa and SiHa cells. Recombinant human IL-8enhanced the viability and reduced the apoptosis in HeLa and SiHa cells. HeLa and SiHa cells cultured in1%oxygen showed increased viability and apoptosis, and the former effect could be partly reversed by anti-human IL-8neutralizing antibody.CONCLUSION:This data suggested that IL-8secreted by cervical carcinomas cells induced by hypoxia can stimulate the viability of cervical carcinoma cells in an autocrine dependent manner, and contribute to the pathogenesis of cervical cancer.