The Protective Effect Of Ursolic Acid On Human Umbilical Vein Endothelial Cells Induced By Ox-LDL Oxidative Damage And Itsmechanism

Objective:To investigate the protection of Ursolic Acid (Ursolic Acid, UA) on human umbilical vein endothelial cells (HUVECs) oxidative damage induced by ox-LDL, and To explore whether the effect plays through Keap1-Nrf2-ARE signaling pathways.Methods:HUVECs were Cultured for80%cell growth density and divided into six groups:group I, control group without; group II HUVECs stimulated with ox-LDL(20mg/L) in endothelial basal medium for24hours; group III, HUVECs treated with LDL(20mg/L)and UA(1.5μmlo/L) in endothelial basal medium for24hours; group IV HUVECs treated with ox-LDL(20mg/L) and UA(3.0μmlo/L) in endothelial basal medium for24hours; group V, HUVECs treated with ox-LDL(20mg/L) and UA(6.0μmlo/L) in endothelial basal medium for24hours; group VI, HUVECs treated with ox-LDL(20mg/L)and Vitamin E (200mg/L) in endothelial basal medium for24hours. Cell absorbance value was measured by MTT assay and the cell survival rate was calculated. Expression of Keap1、Nrf2and HO-1mRNA were determined by reverse transcription-polymeras chain reaction (RT-PCR). Expression of Keap1、Nrf2and HO-1protein were determined by Western blot.Results:Control group, ox-LDL group, the ox-LDL+low concentration ursolic acid group, ox-LDL+the middle concentration ursolic acid group, the ox-LDL+high concentrations of ursolic acid group, ox-LDL+vitamin E group, two groups in terms of cells absorbance value, cell survival, HO-1mRNA and protein expression, Nrf2mRNA and protein expression in turn increased (p<0.05); Keap1mRNA and protein expression in turn reduced (p<0.05).Conclusion:Ursolic acid inhibits human umbilical vein endothelial cells (HUVECs) oxidative damage induced by ox-LDL through Keapl-Nrf2-ARE signaling pathways.