Annexin A5Expressed In Colon Cancer Patients Serum And Its Correlation With Function Of Colon Cancer Cell Biology Research

Research backgroundColorectal cancer is a common malignant tumor of digestive tract diseases, worldwide incidence ranked third in male patients, female patients is higher which ranked second of malignant tumors.However, for the early diagnosis of colorectal cancer rate is not high, many patients come to visit is in progress. At present, for colorectal cancer diagnosis such as CEA, the most widely used tumor markers are not high sensitivity and specificity. We need some more sensitive and specific tumor marker.Annexins are a group of calcium dependent phospholipid binding protein, is found in most eukaryotic cells and the expression of rich. The annexin family members have been found associated with a variety of tumors with Annexin, and A5as the annexin family in the largest and most widely distributed one, its clinical significance and basic research at home and abroad, but few reports.The purpose of the studyThrough the verification of Annexin A5and colon cancer whether there is a correlation, and further study the effect of Annexin A5on human colon cancer cell line function, and preliminary study of Annexin A5involved in the apoptosis of colon cancer cells. Annexin A5as the auxiliary tool for clinical diagnosis and treatment of early colorectal cancer and provide a theoretical basis.Research methods1、Through the change of the staging of Annexin A5serum ELISA and detection of Annexin A5in colon cancer.2、From four strains of colon cancer cell lines were screened Annexin A5highest expression cell line, the lentiviral vector and RNAi interference to silence the expression of Annexin A5in the cells to observe the change of funtions of tumor cell adhesion、invasion、migration、apoptosis、cell cycle and proliferation.3、Using Western blot, RT-PCR Annexin A5involved in the mechanism of apoptosis of colon cancer.The research results1、In TNM stage Ⅲ/Ⅳ colon cancer patients, the serum level of Annexin A5was significantly higher than that of TNM Ⅰ/Ⅱ, also significantly higher than that in the normal control group, and its expression closely related to lymph node metastasis.2、RKO colon cancer cell line Annexin is the highest expression of A5. Annexin A5silencing of RKO colon cancer cell proliferation and colony formation rate, apoptosis rate decreased, and G2/M block, but the invasion, migration and adhesion ability has no effect.3、The Annexin A5after the silence of RKO colon carcinoma cells Bax mRNA expression, protein expression was no significant difference; all increased by the amount of expression of mRNA and Bcl-2protein; expression of phosphorylated Erkl/2protein increased.The conclusion of the study1、Colon cancer patients serum CEA specificity, sensitivity and preliminary experiments and the results reported in the literature are approximately the same, the sensitivity and specificity of Annexin, A5in colorectal cancer patients was higher than that of CEA.2、Annexin A5is highest expression in poorly differentiated colorectal cancer cell line RKO, and consistent with the high expression of A5in the staging of poor carcinoma trend.3、Annexin A5in tumor cell biology experiments promote tumor cell apoptosis, inhibition of proliferation, but the underlying mechanisms in the serum of cancer patients with high expression of both the need to further clarify the phenomenon.4、Annexin A5related apoptosis pathway involved in Erkl/2, and promote the increased ratio of Bcl-2and Bcl-2/Bax, the detailed mechanism requires further confirmation experiment.