Exploration Of The Impact And Mechanism Of Art On Prolifer-Ation Of Chmoresistant Adenoid Cystic Carcinoma Nacc-Ddp Cell Line And Its Pariental Cell

Objective:To explore the effect of artesunate （ART）on proliferation of adenoidcystic carcinoma NACC and NACC-DDP cells; and investigate the possibilityof ART reversing multidrug resistance of NACC-DDP cell.Method:（1）The effect of ART on the proliferation of NACC and NACC-DDPcells was determined by MTT.（2）The expression of c-Myc and ABCG2was tested by qRT-PCR andwestern blot respectively.Result:（1）The cell proliferation capability of NACC and NACC-DDP cells inART group（150~600μM）was significantly inhibited compared with the ARTfree control group by MTT.（2）The expression of c-Myc and ABCG2mRNA of NACC and NACC-DDP cells was reduced by ART（150~600μM）.（3）The expression of c-Myc and ABCG2protein of NACC andNACC-DDP cells was reduced by ART（150~600μM）.Conclusion:ART can decrease the proliferation of NACC and NACC-DDP cells.Molecular level results show that ART can reduce the expression of cell growthfactor c-Myc. The results coincide with the results of cellular level. In addition,ART can reverse multidrug resistance of NACC-DDP cell, suggesting that ARTmay become a new idea of clinical treatment of chemotherapy tolerate humanadenoid cystic carcinoma.