Expression And Biological Function Of Breast Cancer Metastasis Inhibitory Factor (BRMS1) In Human Breast Cancer

Objective:The expression of breast cancer metastasis inhibitory factor (BRMS1) was investigated by the method of PCR in human breast cancer cell lines of MDA MB-231and MCF-7. The biological functions of BRMS1on migration and invasion of breast cancer cells and mechanism of action were investigated by cell functional assays. The correlation between the expression of breast cancer metastasis suppressor1(BRMS1) mRNA and prognosis of breast cancer patients was studied through the analysis against the clinical and pathological indicators of the patients.Methods:Plasmid (MDA231PEF, MCF-7PEF) was transformed by being cloned into E. coli, then the correct structure was selected and amplified through PCR. RNA was extracted from MDA MB-231and MCF-7cell lines and quantified. Then reverse transcription was done with cDNA analyzer. Part genes of BRMS1were knocked down through the PCR assay with the ribozyme added. BRMS1with part genes knocked down (MDA MB231brms1kd, MCF7brmslkd) was cloned into E. coli, then was amplified and purified. The expression of breast cancer metastasis inhibitory factor (BRMS1) was investigated by the method of PCR in human breast cancer cell lines of MDA MB-231and MCF-7.BRMS-1gene transcript was quantified in breast cancer sample tissues and analyzed against the histological and clinical patient outcome. Human breast cancer cell lines, MDA MB-231and MCF-7were used to genetically-modify the expression of BRMS-1and test for biological responses following BRMS-1modifications. Key candidate signal pathways, influenced by BRMS-1were also explored. The expression level of transcription of breast cancer metastasis suppressor1(BRMS1) in human breast cancer was assessed quantitatively through the analysis against the clinical and pathological indicators of the patients.Results:There was an obvious expression of breast cancer metastasis inhibitory factor (BRMS1) in human breast cancer cell lines of MDA MB-231and MCF-7, and the expression was weakened when part genes of BRMS1were knocked down. Knockdown of BRMS1gave both cells a faster cell growth rate, rapid pace of cellular migration and invasion, compared to respective wild-type and control cells (p<0.05). Blocking phospholipase-Cy (PLCy) had a significant influence on the BRMS-1-induced cell migration. Significantly low levels of BRMS1were observed in patients with high-grade tumors (p=0.12), in patients with distant metastasis (p=0.05) and those who died of breast cancer (p=0.0037). In addition, patients with low levels of BRMS1had a significantly shorter overall survival (p=0.035).Conclusion:There were expression receptors of breast cancer metastasis inhibitory factor (BRMS1) in human breast cancer cell lines of MDA MB-231and MCF-7. This is likely to be occurring via its influence on the invasion and migration of breast cancer cells. BRMS1acts as an cellular invasion and migration inhibitory molecule, an action likely involving the PLCy cell signalling pathway. BRMS-1is inversely-correlated with disease progression and patient survival.