Orally Long-term Administration Of Acarbose Impacts The Age Effects On Levels Of Synaptotagmin Ⅰ And GFAP In Dorsal Hippocampus Of Mice

Background With the acceleration of the aging population, how to delay aging and age-related decline in cognitive function, so as to improve the quality of life of theelderly has become a research hotspot. Synaptotagmin (Syt) Ⅰ belongs to thepresynaptic vesicle protein, which involved in the regulation of synaptic vesicleexocytosis fast synchronization. The current study found that the content of SytⅠindorsal hippocampus in sencescence-accelerated prone mouse (SAMP)8was elevated,and was linked to age-related decline of learning and memory. Glial fibrillary acidicprotein (GFAP) is the main protein of cerebral astrocytic intermediate filament, and isthe specific biomarker, its expression tends to increase with age. A large number ofstudied suggest that astrocytes is closely related to cognitive function, especially with avariety of age-related neurodegenerative diseases, such as Alzheimer’s disease,Parkinson’s disease. Acarbose, an α-glucosidase inhibitor, orally long-termadministration of acarbose could stable fasting and preprandial glucose levels, andrestrain the formation of advanced glycation end products. It also has the effect ofpreventing and delaying the occurrence and development of age-related diseases, suchas cardiovascular disease and type2diabetes. These suggested that acarbose may haveanti-aging effect.Objective①To explore the effects of aging on the contents of Syt I and GFAP indorsal hippocampus of senescence-accelerated prone mouse (SAMP)8.②To explorethe effects of orally long-term administration of acarbose on the content of Syt I andGFAP in the Dorsal Hippocampus in SAMP8Mice. Methods Experimental animal were randomly divided into elderly group and acarbosegroup. All mice were free diet. Acarbose group mice to start feeding acarbose from3months old, then add a group of3-month-old mice as young group when the current twogroups mice were9months old.After the behavioral detection task of three groups mice,the contents of Syt I and GFAP in dorsal hippocampus were detected throughimmunohistochemical method.Results①Among all experimental mice, the expressions of Syt I and GFAP weredetected in CA1and CA3subfields and dentate gyrus of the dorsal hippocampus.②Therelative amount of Syt I in the elderly control group was significantly higher than that inthe young control group in each subfields of the dorsal hippocampus (Ps<0.05). Theacarbose treated mice had significantly lower level of Syt I than that in the elderlycontrol mice in each stratun of CA1and CA3subfields, stratum granulosum as well asmolecula of dentate gyrus of dorsal hippocampus (Ps<0.05).③The relative amount ofGFAP in the elderly control group was significantly higher than that in the youngcontrol group in each subfield of the dorsal hippocampus (Ps<0.05). The acarbosetreated mice had significantly lower level of GFAP than that in the elderly control micein CA1and CA3subfields (Ps<0.05).Conclusion①For SAMP8mice, the contents of Syt I and GFAP were varying degreesof age-related increase in each subfield of dorsal hippocampus.②Orally long-termadministration of acarbose could reduce the aging effects of Syt I and GFAP in thedorsal hippocampus in the SAMP8mice.