The Effect Of Bacterial Culture Results After Different Cervical Processing Methods

Objective To compare the effect of bacterial culture results after different cervical processing methods.Methods80patients of pelvic inflammatory disease, bacterial vaginosis, vaginal cleanliness degree III-IV, according to the different different cervical processing methods, were divided into five groups. Group A:to use directly sterile swabs taken cervical secretions; Group B:to take sample after dry sterile cotton ball wiping cervical; Group C:to take sample after normal saline and dry sterile cotton ball wiping cervical successively; Group D:to take sample after iodophor wiping cervical; Group E:to take sample after iodophor, saline and dry sterile cotton ball wiping cervical successively. The above five groups were to take vaginal secretions for bacterial culture at the same time. To observe blood culure medias after18-24hours. According to the different colonies, to pick different colonies for Gram stain, and identification of strains in culture medias. To compare strains of cervical secretions bacterial culture after different cervical processing methods, and compare them with strains of vaginal secretions bacterial culture in comparisonResults Strains of bacterial culture of Group A,compared with vaginal secretions bacterial culture, the difference was no statistically significant(5.31±1.62,6.06±0.68, P>0.05); Strains of bacterial culture of Group B,compared with vaginal secretions bacterial culture, the difference was statistically significant(3.88±1.09,6.19±0.66, P<0.001); Strains of bacterial culture of Group C, compared with vaginal secretions bacterial culture, the difference was statistically significant(3.38 ■1.20,6.06±0.57, P<0.001); Strains of bacterial culture of Group D, compared with vaginal secretions bacterial culture have significant difference(0.69±0.48,6.06±0.44, P<0.001); Strains of bacterial culture of Group E, compared with vaginal secretions bacterial culture have significant difference (3.25±0.45,6.13±0.62, P<0.001). Five groups of vaginal bacteria cultures were no significant difference (F=0.139, P=0.967). Strains of bacterial culture of Group A, compared with cervical secretions of other four groups had statistical difference(P<0.05); Strains of bacterial culture of Group B, compared with Group C had no statistical difference(P>0.05), compared with Group D had statistical difference (P<0.05), compared with Group E had no statistical difference (P>0.05); Strains of bacterial culture of Group C,compared with Group D had statistical difference (P<0.05), compared with Group E, had no statistical difference (P>0.05); Strains of bacterial culture of Group D, compared with Group E had statistical difference (P<0.05).Conclusion Before taking cervical secretions bacterial culture is necessary to preprocess the cervical mouth; Simple processing for cervical mouth, for example sterile cotton ball wiping, can reduce the vaginal microbial contamination.