Effect Of SiRNA Targeting HDAC1Gene On Proliferation, Apoptosis, In Breast Cancer MCF-7Cellcancer Cells In Vitro

ObjectiveTo investigate the effect of silencing histone deacetylases1(HDAC1) geneby RNA interference on the proliferation, apoptosis in breast cancer MCF-7cellline.Methods1. Cultivate human breast cancer MCF-7cell line, select the logarithmicgrowth phase cells. The optimal segment targeting HDAC1gene was designedand transfected into breast cancer MCF-7cells by Lipofectamine TM20002．HDAC1mRNA and protein in the transfected cells were detected byRT-PCR and Western blotting, respectively3.The growth inhibition of breast cancer MCF-7cells was evaluated byMTT assay4. apoptosis were evaluated using cell counting kit and flow cytometry Results1. breast cancer by siRNA interference MCF-7cell, HDAC1mRNAexpression quantity in siRNA transfection group and blank control group,negative control group respectively for（0.150.02）（0.990.23）（0.830.11）,lower than that of blank control group and negative control (P <0.05)2. Western blotting showed HDAC1protein expression quantity alsodecreased obviously, the most obvious to siRNA interference group (P <0.05).3. determined by MTT method to detect displayed in cells after transfection,siRNA transfection group of cells in24,36,48,72h absorbance value of（0.4530.025）、（0.763±0.036）、（1.2140.044）、（1.5110.024）. Visibletransfection cell growth inhibition after24h, inhibiting effect graduallyincreased with the increase of the time (P <0.05), and other growth differencesbetween the two groups4. After silencing HDAC1transfection, the proportion of G1phase and Sphase and G2phase cells respectively （59.62.97)%,(3.50.53)%,(36.91.43)%, S phase proportion reduced (P <0.05), the G1proportionincreased（P <0.05)Conclusion1. HDACl siRNA can efficiently inhibit the expression of HDAC1of breastcancer MCF-7cells2. After silencing HDAC1, can change the cycle and the proliferation of breastcancer cells.