Study On Improper Modification Of Histone Acetylation, Methylation And Alteration Of Regulating Cell Cycle In Diffuse Large B-cell Lymphoma

Objective To investigate the expression of histone acetylated H3, H4, methylated H3K4, H3K9 and P53, P21, cyclin A in diffuse large B-cell lymphoma (DLBCL). We study the pathogenesis of DLBCL.Methods The expression of histone acetylated H3, H4, methylated H3K4, H3K9 and P53, P21, cyclin A were examined by S-P immunohistochemical technique in lymphoid tissue of 36 patients with DLBCL and 16 cases with proliferative lymphadenitis.Result The histone acetylation of H3,H4 were lower expression than that in proliferative lymphadenitis. The high expression rate was 19%,22% (p<0.05). Histone methylated H3K4 was lower expression (19%) (p<0.05) and H3K9 was higher expression (42%) (p<0.01).The P21,P53 and cyclin A positive rate were 33%,56% and 61%, which were higher than that in proliferative lymphadenitis (p<0.01 and p<0.05). There is a positive correlative expression between the global histone acetylation of H3, H4 and histone methylation of H3K4. The expression of P53 was significant negative correlation with histone acetylation of H3 and H4.Conclusion Improper modification of histone acetylations and methylations may play an important role in pathogenesis in DLBCL. It may induce the carcinogenesis by alteration of regulating the cell cycle.