Electrospinning Prepared And Characterization Of Neural Specific Nano Fiber Material With PLGA And Carbon Nanotubes Loading Chinese Medicine Catalpol

Objective: We also try to investigate the effection of nano fibers’solid compositionon micro morphology, diameter distribution, porosity and mechanical properties.Thegrowth situation of nerve cells on the material was observed, and the effect of fibermaterial which was loaded with traditional Chinese medicine catalpol on differentiationof human adipose derived mesenchymal stem cell was studied.Method: The experiment was divided into three parts.(1)The electrospinningtechnological parameter which manufacture the smooth and without beaded fibermorphology was determined, by preparation of single PLGA component nano fiber. Sixkind of different carbon naonotubues’ concentration fiber was manufactured based onthe technological parameter before. The microstructure of material before was observedby scanning electron microscope.(2) The fiber diameter distribution (Image J) andporosity (Origin9.0) of different CNTS concentrations material was measured bydifferent software. The material was cut into5x1cm strip for tensile failure experimentby Instron5948tensil tester, obtained three physical quantities which is young’smodulus,yield strength and ultimate strain of7kinds material including15%PLGAfiber for material mechanical description.(3) The nerve cell growth and survivalsituation was observed by calcein-PI live cell staining and β-tubulinⅢ-GFAPimmunefluorescence staining at the1day,4day,8day three time point which after the extractedrat brain nerve cell was planted on material surface. The growth and differentiation’ssituation of fourth generation AMSC after be planted onto drug loaded fiber material surface which contain Chinese medicine catalpol, PLGA15%and CNTS0.2%.Results:1.The electrospinning process parameter of preparation to continuous andsmooth surface fiber was confirmed as concentration of PLGA15%, loading voltage15kv, receiving distance10cm, solution flow rate1ml/h.2.15%PLGA contain differentcarbon nanotubes concentration (0.1%,0.2%,0.3%,0.5%,0.7%,1%) can also preparedto fiber structure material.3. Carbon nanotubes component which in low concentrationgroup (0.1%,0.2%) was carried on single fiber, increasing the fiber diameterdistribution, porosity and anti external mechanical properties. Carbon nanotubescomponent which in medium concentration group (0.3%,0.5%) was got into carbonblock structure which lead to fiber decreased, causing fiber diameter, porosity andmechanics of material resistance decreased. Carbon nanotubes component which in highconcentration group (0.7%,1%) was formed web structure formation, fiber diameterand porosity increased by viscosity of fiber, material mechanical properties decreasedbut flexibility increased.4. Neuronal and glial cell was not suitable growth on lowconcentration group, and can survive early planted but cannot growth to8day ofneuronal maturation. High concentration group material can carry nerve cells withnormal growth process and not interfere with glial cells growth.5. PLGA15%andCNTS0.2%loading Chinese medicine catalpol can morphological differentiate theadipose derived stem cell to nervous.Conclusion: Conclusion: The nano fiber material which includs carbon nanotubescan be used as nerve cell culture biological material, and can build drug deliverymaterial system by loading Chinese medicine to derive stem cell neural differentiation.The carbon nanotubes’forming mode which followed different concentration in fiberstructure was also explained.