Studies On Extracting Technology For Anti-tumor Components And Its Effect Of Solanum Lyratum

The extraction method of Solanum lyratum Thunb. employed a L₉（3⁴） orthogonal design. The optimal extraction method was 50% EtOH, 3 times extraction and every time for 1 hour. The result of the purification method was macroporous resin/extract ratio 3:1, sample concentration 2g·ml^-1 and 4 times vehicle volume.The optimal extract was introduced to the anti-tumor reseach. In vitro cultured human cervical carcinoma cell HeLa, human hepatoma cell Bel-7402, human gastric adenocarcinoma cell SGC-7901, human fibrosarcoma cell HT1080 and human nasopharyngeal carcinoma cell kB were chosen as research models. The results showed that BY significantly inhibited tumor growth in vitro. In vivo research used model mice transplanted either hepatoma H22 or tumor carneus S180. The results showed that BY significantly inhibited the 2 kinds of tumor, the inhibition rate were over 40%. For the investigation of the effects of BY on immunologic function, mice were given cytoxan （CTX） to form a immune suppression model. Then BY were found remarkably enhanced T lymphocyte proliferation and NK cell activity, and inhibited leucocyte decreasing in these models. Another research on tumor-bearing mice exhibited that BY greatly improved the phagocytize function of mononuclear macrophage, but could not raise the serum erythrocytolysin level dramatically. An alliance dose of BY and CTX on mice bearing hepatoma H22 resulted in significantly lower tumor weight than a single dose of CTX, indicating BY’s synergistic effect with CTX.Visible apoptotic body were detected in BY treated Hela cell afer fluorescein stain with acridine orange （AO）. Flow cytometry results showed that subdiploid peak （Sub-G₁） was detected, and was time and concentration dependant. This implied that BY might induce apoptosis.