The Research About The The Effects Of Fluvastatin And Emodin On Expression Of MCP-1 On Diabetic Rats Kidney

ObjectiveOur study aims to investigate the effects of fluvastatin and emodin on expression ofMCP-1 on diabetic rats models and compare the regulating and protecting effects ofemodin and fluvastatin on renal injury in diabetic rats.MethodsOne hundred and twenty sprague-dawley rats were randomly divided into sixgroups:normal control group (N group),diabetic control group (D group),D+fluvastatingroup (F group),D+emodin group (E group),D+fluvastatin combined with emodin group(FE group).The diabetic rats models were induced by the single injection ofstreptozotocin (STZ) via coccygeal vein.The levels of blood glucose, plasma cholesterol、serum BUN、serum creatinine and 24 hours proteinuria analysis were measured bybiochemistry method.The mRNA and protein levels of MCP-1 in renal cortex weremeasured by reverse transcription polymerase chain reaction (RT-PCR) andimmunohistochemistry.Results(1) The comparison in blood glucose、plasma cholesterol、serumBUN、serum ereatinine: An experimental rat model of diabetes lnellitus wassuccessfully induced by one intravein injection of streptozotocin at a dose of 60 mg/kg andthe blood glucose was higher than 16.7mmol/L and maintained for 8 weeks.The level ofblood glucose in all diabetic treated groups,including F、E、FE groups,didn’t have statisticaldiscrepancy, which indicated that fluvastatin and emodin had no effect on the blood glucosemetabolism.The levels of plasma cholesterol in all diabetic rats were rising since the forthweek.The levels of serum BUN in all diabetic rats were also rising since the secondweek,but a part of groups have no statistical discrepancy.The levels of serum creatininewent up gradually along with the course of diseases,while the increasing of serumcreatinine in the diabetic treated groups was slower than the diabetic control group.(2) The comparison in 24h proteinuria: The 24h proteinuria of all diabetic ratswere increasing since the second week.The 24h proteinuria of diabetic rats in FE group was lower than that in diabetic control group in the forth and eighth weeks,while the F and Egroups were lower than the diabetic control group in the eighth week.(3) The comparison in immunohistochemistry:A significantly higher level ofprotein expression of MCP-1 was observed in all diabetic rats in the second、forth andeighth weeks than the normal group rats.The level of protein expression of MCP-1 indiabetic treated rats(including F、E、FE groups) was lower than the diabetic control group.Among the three groups of F2,E2 and FE2, FE2 and E2 group had statistical discrepancy;and the same as FE4 and F4 group; FEB and F8 or E8 had statistical discrepancy.(4) The comparison in RT-PCR: A significantly higher level ofmRNA expressionof MCP-1 was observed in all diabetic rats in the second、forth and eighth weeks than thenormal group rats.The level of mRNA expression of MCP-1 in diabetic treated groupsrats(including F、E、FE groups) was lower than the diabetic control group. Among the threegroups of F2,E2 and FE2, they hadn’t statistical discrepancy; and the same as FE4 and F4or E4 group; FE8 and F8 had statistical discrepancy.ConclusionThis research show that fluvastatin and emodin have good effect on DM model ratskidney.They may downregulate MCP-1 expression and reduce mononuclear macrophagegathering on DM model rats kidney to slow the progress of DN.and the combination of thefluvastatin and emodin can take synergistic action partly.