Effects Of TFA On The Cell Proliferation And Expression Of Monocyte Chemoattractant Protein-1 In Human Renal Mesangial Cells

Background and aims: Chemokines recruit and activate leukocytes from circulation to inflammatory sites has been thought as an important early event in the development of glomerulonephropathies. Similarly, infiltration and activation of specific monocyte/macrophages, in glomerulus have been implicated in the development of the early stage of diabetic nephropathy (DN). Chemokine such as monocyte chemoattractant protein-1 (MCP-1), has been thought as the candidates to attract respective target cells and activate them in the initial of glomerulonephropathies. More and more evidence show that advanced glycosylation end products (AGEs) is one of the important injury factors involved in the development of chronic complications of diabetes mellitus (DM) including DN. The present study aimed to evaluate the role of interaction of AGEs with chemokines in the development of DN through investigating the effects of AGE-BSA on the expression of MCP-1 and cell proliferation of cultured human renal mesangial cells (HRMC); the inhibitory effects of total flavonoids Abelmoschus manihot(TFA)、total flavonoids Abelmoschus manihot-rat serum(TFA-RS) and Rosiglitazone on the expression of MCP-1 induced by AGE-BSA.Methods: The serum contained with TFA which was prepared by Chinese herbal medicine serum pharmacological approach. The cultured HRMC were incubated with 1640、BSA、AGE-BSA (200μg/ml)、normal serum from rats(1.25%、0.625%、0.313%)、AGE-BSA＋Rosiglitazone 100μM/L、AGE-BSA＋TFA-RS(1.25% 0.625%、0.313%)、AGE-BSA＋TFA ( 200、100、50pμg/ml ). Proliferation of HRMC was measured by the method of methyl thiazolyl tetrazolium (MTT). The supernatant was collected and stored at -70℃, and the cells were harvested to extract total RNA. The supernatant concentrations of MCP-1 were quantified using ELISA. The mRNA levels of MCP-1 of conditioned HRMC were evaluated by semi-quantity RTopCR. The results were showed as means±standard deviation and the data were analyzed with SPSS 10.0 statistical package.Results and Conclusions: TFA attenuated the proliferation and the expression of MCP-1 mRNA and protein in HRMC.