Effects Of Abelmoschus Manihot (L.) Medic On Proliferation And Gene Expression Of PPAR-γ In Glomerular Mesangial Cells

Diabetic nephropathy is a common chronic microvascular complication in diabetes, and it is also the most common type of secondary kidney disease. Its main pathological change is excessive accumulation of extracellular matrix in glomerular mesangial cells, and the late development is glomerulosclerosis. There are many factors for this disease, such as hyperlipidemia, hyperglycemia, hypertention, inflammation and insulin resistance. Currently hyperlipidemia is highly concerned, and is considered to be an independent risk factor for glomerulosclerosis. Therefore, improvement of glucose and lipoprotein decomposition for diabetic patients is very important for the prevention and treatment of diabetic nephropathy. PPAR-γis a member of nuclear hormone receptor super-family, and is highly expressed in liver, kidney, adipose tissue and cartilage. Activated by specific agonist, PPAR-γcan regulate target gene expression at transcription level. PPAR-γis involved in energy metabolism and lipocyte differentiation, and its relationship to kidney disease has become a hot issue. Many scholars believe that PPAR-γcan be used as the target site for the treatment of the urinary system disease.Pharmacology study has suggested that the Abelmoschus manihot (L.) medic has the effects of anti-imflammation, antilipemic, eliminating the oxygen free radical, etc. A main component of it -Quercetin, can inhibited the growth of mesangial cells and reduce accumulation of extracellular matrix. The objective of our study is to discuss action mechanism of Abelmoschus manihot (L.) medic to diabeti nephropathy by evaluating effects of Abelmoschus manihot (L.) medic on proliferation and gene expression of PPAR-γin glomerular mesangial cellsIn this experiment, Abelmoschus manihot(L.)medic played the role in preventing and treating glomerulosclerosis by inhibiting the proliferation of mesangial cells and controlling immunizing inflammatory reaction. MTT and RT-PCR were used to evaluate the effects of Abelmoschus manihot (L.) medic on proliferation of mesangial cells and PPAR-γgene expression at mRNA level respectively. We reached the following conclusions: 1. PPAR-γplays the role in preventing and treating of glomerulosclerosis by inhibiting the proliferation of mesangial cells and controlling immunizing inflammatory reaction. 2. High glucose can down-regulate mRNA expressions of PPAR-γby activating P38MAPK pathway. Disruption of p38 MAPK pathway or up-regulation of expression will become an important tool for therapy of diabetic nephropathy. 3. Abelmoschus manihot(L.)medic can antagonize the down-regulation of high glucose to the PPAR-γmRNA expression in a concentration-dependent manner. We have worked out the hypothesis that Abelmoschus manihot (L.) medic may play the role through inhibiting the PKC-P38MAPK pathway. 4. Abelmoschus manihot(L.)medic can inhibit the proliferation of mesangial cells, and with the increased concentration, its function is increased, maybe due to the up-regulation of PPAR-γexpression.