Aqueous Enzymatic Extraction Sorbifolia Seed Oil And Biodiesel Preparation Step

Shinyleaf yellowhorn (Xanthoceras sorbifolia bunge) is mainly produced in china. Root can go deep into soil, and have strong adaptability to soil. It can resist cold and drought. Especially,it can endure safe wintering in extremely low-temperature environment. In precipitation deficit place there is Shinyleaf yellowhorn. It classified as tree species of soil and water conservation. In the ecological management, it has a broad application prospect. Shinyleaf yellowhorn’s fructicative period and full baring period is long. The kernel contained oil amount more higher. It is a precious peculiar species in China and a kind of superior woody oil crop in the north of China. It has abundant oil resource, that provides broad prospects for the development of Shinyleaf yellowhorn.The focus of this study is Shinyleaf yellowhorn oil resource. The aims of this study are as follows:1. Aqueous enzymatic extraction(AEE) of oil fromShinyleaf yellowhorn kernels was performed. Using response surface methodology(RSM) optimization design method;2. A conventional Soxhlet cxtraction(SE) was carried out to compare the oil extraction performances by AEE method. It include physical and chemical properties and fatty acid compositions of kernel oil.3. Based on Shinyleaf yellowhorn kernels as raw material, the transesterification to biodiesel catalyzed by solid alkali was study. The research content as follows:Using AEE of oil from Aqueous enzymatic extraction(AEE) of oil from Shinyleaf yellowhorn kernels was performed. Selection of cellulaes and alkaline protease enzyme for experiment, the effects of pH, amount of enzyme, temperature and solid/liquid ratio were discussed by single experiments. Using design expert software, the four AEE parameters namely solid/water ratio, extraction time, cellulose amount, Alkaline protease amount, were optimized by response surface methodology. The optimal extraction conditions for oil yield were solid/water ratio, extraction time, cellulose amount, Alkaline protease amount as1:6.07,4h,3%(w/w),0.38%(w/w). Under the optimum conditions, the experimental oil extraction yield was39.68%. The error between the actual value and the predicted value is0.1%.A conventional Soxhlet extraction(SE) was carried out to compare the oil extraction performances by AEE method. The AEE of oil has grain smell, no solvent, AEE is safe and healthy. Acid value(AV), peroxide value(POV) and saponification value iodine value showed insignificant difference. The fatty acid composition analysis was performed with GC-MS for the different shinylaef yellowhorn kernel oil obtains by the procedures of SE and AEE.Both oils were very abundant in the unsaturated fatty acids and obtain relatively low content in the saturated fatty acid.The content of PUFA(9,12-octadecadienoic) of AEE extracted oil was higher, while the9-octadecanoic was relevant lower. So, the AEE was more efficient method to extract PUFA than the SE process. The acid value of Shiny leaf yellowhom kernels oil is about lmg/g. And this kind of oil can be directly for the preparation of biodiesel without acid treatment. Based on Shinyleaf yellowhorn kernels as raw material, the transesterification to biodiesel catalyzed by solid alkali was study. The effects of solvent ratio, catalyst dosage amount, temperature and reaction time were discussed by orthogonal experiments. Under the optimal conditions of solvent ratio4:1, catalyst dosage amount6.5%, temperature60℃, reaction time2h. Biodiesel yield is was86.79%. The products were analyzed by GC-MS and a mixture of methyl ester. Extraction and Synthesis of biodiesel is in the same process. Then the technology has some advantages of simple operation and lower production cost.