Cytoskeleton And Preparation Of Composite Applications In Drug Screening

It is a vital Screening model system in the drug screening field combined three dimensional cell culture and HPLC. Creating the method of effective screening and selective analysis for the specific and affmitive component groups in traditional Chinese medicine has attracted much more attention from the relevant realm. Many research works been more inclined to accept the fact that the whole cells might be more actual to reveal the interaction of drugs with protein on the surface of cells in vivo, so this kind of method had became a significant branch of traditional Chinese medicine analysis. However, in recent years, three dimensional cell cultures with its unique advantage become a hotspot in this research field.It is necessary to develop a novel method for preparing large volume three-dimensional scaffolds which the pore structure, uniformity, and inter connectivity can be controlled easily. In order to meet the requirements of tissue engineering and drug carrier, we must follow the basic principle that an ideal porous scaffold must have the characteristics of excellent biocompatibility, cytocompatibility, suitable microstructure (pore size and porosity) and mechanical properties.A broad range of natural and synthetic biomaterials are used to fabricate various composite scaffolds to improve their noumenal properties. Composite scaffold materials can concentrate the performance advantages of different compositions and avoid respective defects. Chitosan (CS), silk fibroin (SF) and nanohydroxyapatite particles (nHA) are commonly used materials because of their good biological compatibility. CS degrades quickly and has low mechanical strength, whereas nHA degrades slowly and has high mechanical strength. SF is superior in its biological compatibility and high mechanical strength, however, its rapid degradation has limited its extensive utilization in the biomedical materials field.Composite porous scaffolds have attracted extensive attention in the biomedical materials field. The aim of this research was to prepare a novel tri-component composite porous scaffold and to evaluate its relevant properties. The porous structure was achieved using a porogen (salt) and the pore size was controlled by the size of porogen. To evaluate the characteristics of the tri-component scaffold, three bi-component scaffolds, CS/SF, CS/nHA and SF/nHA, were simultaneously prepared for comparison. The scaffolds were subjected to morphological, micro-structural and biodegradation analysis. Results demonstrated that all of the scaffolds had pore sizes of100-300μm and a porosity of90.5-96.1%. The biodegradation characteristics of all scaffolds meet the requirements of good biomedical materials. The investigation of the mechanical properties showed that the tri-components scaffold has better properties than the bi-component scaffolds. The in vitro biocompatibility with osteoblast-like MG-63cells showed that all the scaffolds are suitable for cell attachment and proliferation, however, the CS/SF/nHA composite porous scaffold is much more effective than the others.In addition to the component materials, the fabrication technique has also been reported to affect scaffold properties. CS can form unique nanofiber structures using freezing-drying methods, and the porosity of the scaffolds can be controlled by the size of the porogen.In order to investigate the practical application of tri-component scaffold in the drug screening, the following test that loading MCF-7cells on the CS/SF/nHA to interact with the cornus extraction had been conducted. The results demonstrated that three kinds of components from cornus extraction can combine properly with cells showed by the chromatograph finger-printing.