Oyster Extract Nutrients Analysis And Effective Part Of Screening

This thesis mainly includes two parts, the nutrition analysis of oyster and its extract, and activity screening about effective part of oyster extract.Section one:the nutrition analysis of oyster and its extract.The fresh oysters were extracted with water at the room temperature, and then the oyster residue was extracted with acetic acid solution of3%. The experimental results of nutrition analysis about the oyster and its extracting solution Oyo, Oy1and Oy2, showed that Oy1, Oy2contained high protein (33%and53%), and were full range of amino acids. It indicated that the contents of protein and amino acid obtained from3%acetic acid solution were obviously higher.Extracting solutions were paragraphed with alcohol. Then the similar components of spectra were merged by UV scanning, and the merged components were signed A-H. Nutritional analysis was carried out on the components which were the remaining after ethanol precipitation. The results showed that Oyo’contained elements K, Mg, Ca, Zn with high content, the content of elements K, Mg, Ca, Cu, Fe, Zn were high in Oy1’, and Oy2’was rich in elements K, Mg, Ca, Mn and Zn. The components B, D, F had hypoglycemic activity after the screening experiment about postprandial blood glucose with normal mice. Through measuring the content of amino acid and glycogen about components B, D and F, the results indicated that the main ingredients of B were protein and glycogen, protein was the main ingredients of D, and F had high glycogen、protein and inorganic salt. So they played a physiological activity may be for one or interaction of several kinds of ingredients.Section two:activity screening about effective part of oyster.Through establishing animal experiment model and giving different oyster extract, explored the therapeutic effect, and screened effective active site by detecting index and observing viscera pathological slice. 1. The experiment of hyperglycemic(1)Through the experiment about postprandial blood glucose with normal mice to screen the components A, B, C, D, E and F, the result showed that components B, D, F (200mg/kg) had an inhibitory effect in a certain extent on postprandial blood glucose within30min of normal mice.(2) The hyperglycemic mice were induced with alloxan solution by intraperitoneal injection, and administrated with components B, D, F in high and low dosage (600mg/kg,400mg/kg), and the positive drug glyburide (100mg/kg) for3weeks. Through the measurement of fasting blood glucose after2weeks and3weeks, it was found that B low dosage (400mg/kg) could significantly reduce blood glucose of model mice, with significant difference (P<0.05). Compared with model group, liver index and kidney index of B low dose (400mg/kg) were significantly decreased (P<0.01), but spleen ratio had no significant difference. The result indicated that oyster extract of B could protect liver, and has a certain role in promoting renal function. Through the observation of pancreatic pathological slices, it was also found that B (400mg/kg) had restoration and protective effects on bata cells, and restoraative function on the islet.2. The experiment of antihyperlipidemicFeed high fat die for12weeks to establish the model of hyperlipidemia, and then give them components B, D, F, G (400mg/kg) and positive drug simvastatin(1mg/kg) when the model of hyperlipidemia was succeed.Test four indicators in serum lipids, calculat the organ index and then make liver pathology slice after five weeks. The results showed that TG level had significant difference (P<0.05) in the group B and positive drug group (simvastatin) which was decreased by35.2%and44.4%respectively, compared with the model group,. The liver weight was slightly reduced in B group, but it had no effect on kidney and spleen weight. Observing the liver pathological slices, the amount of fat vacuoles in cytoplasm about group B had certain decrease, and lower than positive group, compared with model group. All the result indicated that component B could reduce the TG level of plasma in hyperlipidemia rats, and had certain therapeutic effect on fatty liver disease. 3. The experiment of alcoholic liver injuryEach group was intragastric administration for56degrees liquor (12mL/kg/d) to establish a model of alcoholic liver injury, meanwhile prevention group of middle dose was administrated with component F (300mg/kg) from the beginning of the experiment. Give component F with high、middle and low dose (450mg/kg、300mg/kg and150mg/kg), and positive drug Polyene Phosphatidylcholine Capsules (50mg/kg) after7weeks. Test indicators in serum, calculat the organ index and then make liver pathology slice after five weeks. Experimental data showed that, the lipid parameters TG had the extremely significant difference (P<0.01), with a decrease of49.2%in the treatment group of middle does (300mg/kg), and had significant difference ((P<0.01) in the two detection, decreased by53.8%、57.5%in prevention group of middle dose (300mg/kg), compared with model group. The other data had no significant effect. The result indicated that middle does of component F (300mg/kg) had very good prevention and treatment effect on the increase of TG level at the early stage of alcoholic rats, and the effect was good.