Discussion From Caveolin-1 Expression Huoxue Network Piece Wing Pro-angiogenesis Mechanism After Cerebral Ischemia In Rats

Objective:Immunohistochemistry to be adopted to clarify the blood circulation Wing contact tablets on the mechanism of action of angiogenesis after cerebral ischemia, to provide experimental evidence for Chinese medicine treatment of cerebral infarction and target of clinical medicine.Methods:Using MOCA Law modeling, reference to the method of Longa cavity inside bolt, and make improvements, copy the model of the middle cerebral artery ischemic injury. After the modeling, the rats were randomly divided into sham group, model group, blood circulation Rong network chip group, buflomedil group, the experimental group divided into the blood circulation Rong network chip group and phosphate pyridoxal aldehyde small slightly Seoul group, model group, sham group was the control group, n=24rats, postoperative sham group, model group given distilled water orally; Huoxue Rong network chip group, pyridoxal phosphate buflomedil group be drug gavage.24h3d,5d,7d neurological deficit scores and brain tissue infarction size determination TTC staining at the corresponding time points decapitation. Immunohistochemical determination of cerebral ischemia in rats the ischemic area microvascular density (MVD), expression of caveolin-1protein expression.Results:Rat model, promoting blood Rong network chip group and pyridoxal phosphate buflomedil group gradually increased over time neurological score, the statistical significance (P<0.05); sham group, model group, no significant changesthe difference was not statistically significant (P>0.05); the TTC stained determination the infarction size model group at four time points after staining infarct size without significant changes in the statistical analysis (P>0.05). Huoxue Rong network piece with pyridoxal phosphate buflomedil group infarct area in varying degrees narrow and blood Rong network chip group to reduce significantly (P<0.05).Conclusion:Blood Rong network chip can promote an increase in cerebral ischemic infarct zone microvessel density (MVD), significantly raised caveolin-1expression in ischemic cerebral infarction organization, its mechanism of action may promote brain angiogenesis, thereby significantly improving theneurological deficit after cerebral ischemia.