Vancomycin Hydrochloride Cationic Vesicle Preparation And Quality Study Liposomes

Objective:To prepare cationic vancomycin MVLs and investigate their quality.Methods:Cationic Vancomycin MVLs were prepared by double emulsion method and The storing solution of vancomycin was prepared. Established the analysis method of vancomycin in vitro and estimated its specificity, precision and resorption rate.RP-HPLC was used to determine the concentration of vancomycin, encapsulation efficiency and release characteristics In vitro. The formulation and pharmaceutical process were optimized by single factor experiments and orthogonal experimental design with the factor of encapsulation efficiency as the criteria. The liposome morphology was observed by optical microscopy and transmission electron microscopy.The particle size and Zeta potential were determined by Malvern instrument. The Stability was analyzed by dynamic analysis.Results:An HPLC method was established for the assay of vancomycin. The analysis method was precise,simple and reliable for the quality control of Vancomycin. Cationic vancomycin MVLs seemed round and well-distributed, the average particle size and the encapsulation efficiency respectively were3.3um and24.9%. Zeta potential was24.53mv.90.5%of vancomycin was released after11days in normal saline under37℃. Cationic vancomycin MVLs were stored for one month at4℃,which appeared good stability.Conclusion:Cationic vancomycin MVLs had good appearance, high encapsulation efficiency, good stability and significant sustained release properties.