Incubate For HU210 Morphine Induced Calcium Oscillations In The Brain Astrocytes

Glial cells, as a type of nerve tissue cells, are widely distributed in the central nervous system and peripheral nervous system. Along with the further study of glial cells, the current view is that the glial cells in the central nervous system is not only simple play roles in support, insulation, barriers and nutrition, but also play important roles in synapse formation and synapse plasticity, the secretion and uptake of neurotran-smitters etc, therefore to regulate a variety of physiological activities of neurons. In the central nervous system, astrocytes are the largest neumber of cells in glial cells. Neuron-s can generate action potential to express its excitement, and unlike neurons, the excita-bility of astrocytes mainly via rising intracellular free Ca2+concentration ([Ca2+]i) to perform. While astrocytes excited, they can release gliotransmitters that could react on neurons in order to regulate synaptic efficacy and synaptic plasticity. Thus, the calcium signal of astrocyte become a hot topic in recent years.In this experiment, we put the primary cultured cortical astrocytes of Sprague-Da-wley (SD) rat as a model, and morphine incubate the cultured cells in short-term. We trough observing the effect of HU210perfusion on the intracellular calcium signal of cultured astrocytes from rat cerebral cortex in order to understand the effect of cannabi-noid drugs on astrocytes and the effect of morphine on HU210incubation effect.Astrocytes were separated from cerebral cortex of new born Sprague-Dawley (SD) rats (1-3days old) in aseptic conditions. The cultured astrocytes were identified by immunocytochemistry that showed glial fibrilary acidic protein-positive (GFAP). We used Fura-2/AM as Ca2+indicator to detect changes of [Ca2+]i pre-and post-perfusion of HU210in control group, morphine group and naloxone plus morphine group of astrocytes. We also examined the effects of blockage CB1receptor and μ-opioid recept-or on induced intracellular calcium oscillations.The result of experimental showed that stimulated by HU210perfusion,[Ca2+]i of the astrocytes in control group, morphine group and naloxone plus morphine group, were increased obviously, because of in each group, the fluorescent values (F340/F380) of most cells increased significantly. whereas this effect was bolished by the correspon-ding receptor antagonists AM281, and this suggests that HU210could elevate astrocyte [Ca2+]i through the activation of CB1R. While there is a different in [Ca2+]i of the astrocytes between the control group and the morphine group, and this effect was bolished by the corresponding receptor antagonists naloxone, and this suggests that the morphine short-term incubation can affect HU210and this effect can be naloxone inversion.In conclusion, the activation of CB1receptor by HU210can increase [Ca2+]i cul-tured rat astrocytes, morphine can strengthen the effect of HU210and naloxone can r-everse the effects of morphine. The reason for this phenomenon may be due to there is a influence between opioid receptor signaling system and endogenous cannabinoid sy-stem.