Analysis Section White And Red-brown Soil Fuling Chemical Composition

ObjectiveIntends to explore the difference and internal relationship in the chemical constituents between two kinds of Smilax glabra,which’s section are red brown and off-white,and to provide the basis for Smilax glabra of the two section color further analysis and research,the research group determined and analysed the content of total tannin,total polysaccharides and total flavonoids in two kinds of Smilax glabra,separated and extracted the total flavonoids to implement pharmacodynamic test,and study on the characteristic map of Smilax glabra.Method1.Total tannin was determined by UV spectrophotometry,with gallic acid as reference substance and phosphomolybdotungstic acid as chromogenic agent.And investigate concretely the effects as extraction method and developing time and so on,to establish the method for content determination of total tannin in Smilax glabra.2.Determination of total polysaccharide in Smilax glabra was determined with phenol-sulfuric acid by UV spectrophotometry,with glucose as reference substance. And the developer dosage,developing time and the other effects were investigated,and the method for content determination of total polysaccharide in Smilax glabra was established.3.The total flavonoids was determined by UV spectrophotometry at500nm,with rutin as reference substance and NaNO2-Al(NO3)3-NaOH colorimetry.4.Using water extraction and alcohol precipitation method,gelatin precipitated to remove the total polysaccharide and total tannin in extract.And the flavonoids was divided into high and low dose group for pharmacodynamic test.5.HPLC was used to establish the characteristic maps of two kinds of Smilax glabra, and the maps were analyzed and compared to found the difference between them.The chromatographic conditions included C18column(250×4.6mm,5um) with the temperature of25℃,mobile phase acetonitrile-water containing0.05%phosphate acid with gradient elute at the flow rate of0.8ml/min and at the wave length of290nm. Results1.The optimum extraction process of total tannin was ultrasonic extraction with60%ethanol for20minutes.Using tannic acid instead of gallic acid in the recovery test has a better effect.The results show that the content of total tannin in the Smilax glabra with section red brown in Guizhou is higher than the Smilax glabra with section off-white in the other provinces.2.The optimal colorimetric conditions of total polysaccharide were as follows:the dosage of5%phenol and concentrated sulfuric acid were1.0mL and5.0mL,and reaction10minutes at80℃and cooling3minutes in the mixture of ice and water.The results show that the content of total polysaccharides in Smilax glabra with section off-white is higher than the Smilax glabra with section red brown.3.The optimum extraction process of total flavonoids was ultrasonic extraction with methanol for30minutes at the solid-liquid ratio of1:200.The results show that the content of total flavonoids in Smilax glabra with cross section red brown was higher than the Smilax glabra with section off-white.4.The total peak area in the characteristic map of total flavonoids based on the pharmacodynamic experiment is a reference to determine the high and low dose for pharmacodynamic test.5.Characteristic maps of two kinds of Smilax glabra were established,and the similarity of each map is above0.9.It was found that the difference between two through comparing characteristic maps.Conclusion1.It was found that there were some difference in the three types of compositione through comparing the content of total tannin,total polysaccharides and total flavonoids in two kinds of Smilax glabra:the content of total tannin and total flavonoids in Smilax glabra with section red brown were higher than the Smilax glabra with section off-white;while the total polysaccharides in Smilax glabra with section off-white was higher than the Smilax glabra with section red brown.It suggested that it whether can proved in a certain degree the two has some differences in the species because of the differences in the main component. 2.The results of the characteristic map showed that there were some differences in the characteristic peaks between two kinds of Smilax glabra,further indicating a possible difference in varieties.3.It did exist certain differences in chemical constituents between two by comparative the results of the coparative analysis about three types of compositione and the characteristic maps.The difference whether from the environment or the difference of the two genes,the underlying reason of which is worth further research.