Qiliqiangxin Capsule On Chronic Heart Failure And Its Effect On AQP2 Expression In Rat Kidney Function And Mechanism

1ObjectiveTo observe the effect Qiliqiangxin capsule on rats with heart failure after myocardial infarction heart function and plasma AVP, Ang II changes and AQP2, pS256-AQP2, V2R, AT1R protein expression.Discussion Qiliqiangxin capsule on chronic heart failure function and water metabolism in rats and its possible molecular mechanisms.2Method2.1Select50healthy male SD rats (sham group10and made module40). Modules made using the left anterior descending coronary artery ligation model of chronic heart failure were prepared, after4weeks, echocardiography,take LVEF≤45%were randomly divided into three groups:model group, Qiliqiangxin group valsartan group.Administered four weeks after the line changes echocardiography each index.2.2Using radioimmunoassay plasma AVP, Ang II of change.2.3Using immunohistochemistry and western blotting method to detect AQP2, pS256-AQP2, V2R and AT1R expression.3Result3.1And sham compared with the model group EF, FS, SV was significantly lower (P<0.05), LVIDd, LVIDs, EDV, ESV (P<0.05), significantly increased. And the model group compared Qiliqiangxin group and valsartan group EF, FS was significantly higher (P<0.05), LVIDs, ESV was significantly lower (P<0.05), Qiliqiangxin group and valsartan group LVIDd, EDV compared with the model group, although a decreasing trend, but the difference was not statistically significant (P>0.05), Qiliqiangxin SV group and valsartan group compared with the model group, the difference was not statistically significant (P>0.05). Qiliqiangxin group and valsartan group EF, FS, LVIDs, ESV difference was not statistically significant (P>0.05).3.2Show radioimmunoassay test results:Compared with the sham group, model group, the plasma AVP, Ang II was significantly higher (P<0.05); compared with the model group, Qiliqiangxin group and valsartan plasma AVP, Ang II significantly lower (P<0.05); while Qiliqiangxin group and valsartan plasma AVP, Ang II levels considerably, the difference was not statistically significant (P>0.05).3.3Modelgroup AQP2, pS256-AQP2, V2R, AT1R protein expression levels were significantly increased (P<0.05); compared with the model group, AQP2, pS256-AQP2, V2R, AT1R expression Qiliqiangxin and valsartan group were significantly lower (P<0.05), two AQP2, pS256-AQP2, V2R, AT1R expression, the difference was not statistically significance (P>0.05)4Conclusion 4.1Qiliqiangxin capsule on heart failure after myocardial infarction in rats and water retention left ventricular function improvement, and ARB drugs valsartan capsules considerable role.4.2Qiliqiangxin capsules to reduce water retention and the role of inhibiting the expression and redistribution of AQP2and pS256-AQP2reduce the renal collecting duct water reabsorption related.4.3Qiliqiangxin capsule of the kidney AQP2regulation may be through AVP-V2R-AQP2and Ang Ⅱ-AT1R-AQP2way.