The Effect Of Wheat Peptides On Skeletal Muscle Protein Synthesis In Rats With Altitude Training And Its Mechanism

Objective:To explore the effect of wheat peptide supplement on skeletal muscle protein metabolism in the rats with altitude training and its mechanism.Method:The study objects are60clean male SD rats, weight160-180g. After two weeks of adaptive feeding, the rats are randomly divided into6groups, control group (group C, n=10), altitude for control group (group HC, n=10), Exercise training group (group E, n=10), Add wheat peptide+exercise training group (group EW, n=10), altitude training group (group HE, n=10), altitude training+wheat peptide group (group HEW, n=10). Ordinary feeds separatly given according to the grouping requirements of the experiment. The weight of the rats are measured Weekly. Irrigate wheat peptide solution after every training and dose of wheat peptide is500mg/kg.bw. Draw materials on next morning of the last exercise training and keeping fasting. the serum T, Cor content and Pro, Myo, IGF-1and AR content in skeletal muscle are measured.Results:①ompared with group C, Pro contents in skeletal muscle in group E had no significantly reduction (P＞0.05), while Myo contents significantly reduced (P＜0.05). Serum T concentration in group E decline and serum Cor content somewhat rised, but there are no significant difference (P＞0.05). IGF-1and AR content in skeletal muscle in group E significantly reduced (P＜0.05)②ompared with group C, Pro and Myo Pro contents in skeletal muscle of group HC were extremely significantly reduced (P＜0.01). At the same time, IGF-1and AR content in skeletal muscle and serum T content in group HC were significantly lowered (P＜0.05) while serum Cor increase significantly (P＜0.05)③hrough the double factor variance analysis, long-term altitude training can significantly reduce Pro and Myo contents in skeletal muscle of the rats (P=0.05). Simulated altitude training can reduce IGF-1and AR content in skeletal muscle of rats, but no significant difference(P＞0.05), and can significantly reduce Serum T concentration(P＜0.05), and increase serum Cor concentration of rats (P=0.05)④Through the double factor variance analysis, supplement of wheat peptide can significantly increase Pro and Myo level in the exercise training rats (P＜0.01) and can significantly increase IGF-1and AR content in exercise training rats (P＜0.01), and serum T concentration(P＜0.01). Although it can reduce Cor serum level in exercise training rats, but no significant difference (P＞0.05)⑤hrough the double factor variance analysis, hypoxia and supplement of wheat peptide have significant interaction for increasing muscle Pro contents (P＜0.05) in rats with overload training. Although hypoxia and supplement of wheat peptide could make muscle Myo and serum T content in rats with overload training to be further increased (P＞0.05), there are no significant difference, while that have significant interaction for IGF-1and AR to be further increased, reduce serum Cor content (P＜0.05)Conclusions:①Although9weeks aerobic exercise training has no significant influence on Pro contents of skeletal muscle in rats, it can significantly reduce Myo contents of skeletal muscle. Long time endurance training leading to the reduction of IGF-1and AR content in skeletal muscle, may be one of the mechanism for the reduction of protein synthesis in skeletal muscle.②9weeks hypoxia exposure can significantly reduce Pro and Myo contents in skeletal muscle of rats. Long-term hypoxia exposure restrain the secretion of IGF-1, T and reduce AR content in skeletal muscle, promote the secretion of Cor, may be the mechanism that long-term hypoxia exposure reduced protein synthesis in skeletal muscle.③imulate altitude training can significantly reduce skeletal muscle Pro and Myo contents in rat, and altitude training restrain the secretion of T and promote the secretion of Cor, which may be main mechanism that long-term hypoxia exposure reduced protein synthesis in skeletal muscle.④he supplement of wheat peptide can significantly increase Pro and Myo contents of skeletal muscle in the rats with overload aerobic exercise training, and supplement wheat peptide can significantly increase IGF-1, AR content of skeletal muscle and serum T content in exercise training rats, which may be the mechanism wheat peptide promote protein synthesis in skeletal muscle in rats with overload training. ⑤he supplement of wheat peptide can significantly increase simulate altitude training skeletal muscle protein content in rats, and wheat peptide supplement significantly increase IGF-1and AR content of skeletale muscl in rats with simulate altitude training, reduce serum Cor content, which may be the mechanism that the wheat peptide promote protein synthesis in skeletal muscle of rats with altitude training.