| ObjectiveUsing drosophila as a model animal for study the cardiac aging, and by inverse gravity climbing index established movement anti-aging Drosophila model,through the UAS-GaL4binary transgenic systems and movement of the Drosophila gene regulation by Drosophila cardiac function testing system and biochemical markers of aging-related testing, to further explore the functional candidate gene PARP-1sport anti-aging system of the heart, the study provides evidence for functional sports-related anti-aging gene.Metairie and methodsDrosophila31397strains (buy from Bloomington Drosophila Stock Center), arm-Gal4and wild-type w1118strains (Heart Development Research Centre of Hunan Normal University), the collection expanded cultivation within8-12h emergence of male fruit flies2400, and were randomly divided into four groups:1week old quiet control group (younger group),1week exercise training group (younger exercise group),5-week-old sedentary control group (aged control group),5-week-old movement training group (younger exercise group),600/group,20/tube, Flies are housed in a25C incubator with50%humidity and a12-hour light/dark cycle. Motor cultivate cotton plug bottom of the tube to the lower area reserved for8cm as Drosophila sport, exercise frequency by the movement of the device:the use of platform motion (Drosophila motion device independent platform developed by the research group, has obtained national invention patents, patent No.201110349810.4) speed control, exercise frequency is24rev/sec, flies were randomly divided into four groups, one week exercise group and the sedentary group, five weeks in the exercise group and the sedentary group, exercise time is moving2.5h/d, one week exercise group from the collection after the second day of the first week of Drosophila continuous motion five days, five weeks after the exercise group from the beginning of the fifth week of the next day to collect flies continuous motion five days.Results3.1The result of life cycle in DrosophilaThrough the different groups of Drosophila life cycle statistics found in wild-type flies hybridized with w1118-control ratio,1w-w1118-exercise Drosophila average life expectancy (p<0.05), but5w-w1118-exercise instead shortened lifespan of Drosophila (p<0.05), shows a different time in different sports interference effects on Drosophila life, early exercise training can extend the average life span of fruit flies, but instead make exercise training in older flies shorter average life. W1118-control with the ratio of wild-type, parp-RNAi-control group, despite the increase in average life span of Drosophila, but this increase does not have significant difference. And parp-RNAi-control ratio, lw-Parp-RNAi-exercise group flies an average life expectancy (p<0.01),5w-Parp-RNAi-exercise group and the highest lifetime average lifespan of Drosophila no significant changes.3.2The result of climbing ability in DrosophilaThe climbing ability of flies decreased significantly with the age (P<0.01).1w-W1118-exercise which strains of Drosophila climbing ability is higher than the lw-W1118-control (P<0.01). And parp-RNAi-control group than in, lw-Parp-RNAi-exercise group improved climbing ability of Drosophila (P<0.01). The lw-parp-RNAi-control group than1w-w1118-control, although Drosophila climbing ability was increased, but this increase does not have significant differences.3.3The result of heart function in Drosophila3.3.1The result of heart rate in DrosophilaBy testing found that the Drosophila heart rate increases with age gradually decreasing trend (P<0.01), but after Parpl gene interference, heart rate increased with age and decreasing trend eased. 3.3.2The result of heart arrhythmia index in DrosophilaWith the age increasing, the age of five weeks of Drosophila fruit flies over a week heart rhythm arrhythmia index were significantly increased (P<0.01), and one week old wild-type w1118-control group than in the wild-type week old w1118-exercise uneven rhythm group index declined significantly (P<0.01), described in Drosophila week old exercise training can improve the stability of cardiac function. Simultaneously with one week old wild-type w1118-control group than one week old Parpl-RNAi-control Drosophila heart rhythm arrhythmia index also declined, indicating Parpl gene silencing can significantly improve heart rhythm, thereby improving cardiac function.3.3.3The result of heart fractional shortening in DrosophilaWith the age increasing, the wild-type group and the control group, the Drosophila gene silencing groups have reduced ejection fraction, and (P<0.01). And1w-w1118-control group than in,1w-w1118-exercise group flies ejection fraction increased (P<0.05), found by comparing the contraction was mainly due to the decrease in diameter (P<0.05), whereas no significant change in diastolic diameter. And with5w-w1118-control ratio,5w-w1118-exercise group ejection fraction did not change. And1w-w1118-control group than in, lw-Parpl-RNAi-control group ejection fraction increased (P<0.01), with the5w-w1118-control ratio,5w-Parpl-RNAi-control group had no significant ejection fraction changes. And lw-Parpl-RNAi-control group than in, lw-Parpl-RNAi-exercise group flies ejection fraction increased (P<0.05), with the5w-Parpl-RNAi-control group than in,5w-Parpl-RNAi-exercise Ejection fraction increased (P<0.05).3.3.4The result of fibrillations in Drosophila heartWith the age increasing, the fibrillations of cardiac increased in Drosophila(P <0.05). Through exercise intervention,1w-w1118-exercise compared to1w-w1118-control, the fibrillations of cardiac was significantly lower (P<0.05). And after parpl gene silencing, lw-Parpl-RNAi-control compared to lw-w1118-control group, the fibrillations of cardiac decreased significantly (P<0.01). 4. Conclusion4.1By UAS-Ga14system successfully achieved the gene of Parp1low expression, the Parpl gene silence can lead to Drosophila life cycle extension. Meanwhile, in this exercise intensity,1week exercise intervention can extend the average life span of fruit flies, while the5-week-old movement to intervene but to make shorter average lifespan of Drosophila.4.21week exercise intervention can improve exercise capacity in Drosophila, while the5-week-old exercise intervention reduced exercise capacity but to make fruit flies, suggesting that exercise intensity at this time to make aging in Drosophila body to produce non-adaptive, thus affecting the movement capacity. The low expression of genes Parp1late exercise intervention can alleviate exercise capacity due to decreased.4.3exercise intervention can improve cardiac contractility of drosophila, helping to enhance the Drosophila cardiac ejection function.4.4As the age increasing, heart function of Drosophila gradually weakened, the gene of Parpl low expression can significantly improve heart rhythm, thereby improving cardiac function. |
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