Study On Self - Assembly Of Gold Nanoparticles Based On Fluorine And Fluoride And Reversible Agglomeration Of Protein Regulation

The self-assembly of Gold nanoparticles and its application inspires many research interests. Gold nanoparticles can self-assemble into complex structures, including multiple dimensional nano or micro-structures by various driving forces and self-assembly methods. And due to the low toxicity, biocompatibility, unique electrical and optical properties of gold nanoparticles, it is widely used in the fields of biosensor, biocatalysis, cell imaging, drug delivery etc. In the past20years, as the development of molecular biology, more evidences show that carbohydrate is one of the major factors determining the molecular recognition of many physiological and pathological processes.However only a few examples of gold nanoparticles functionalized by carbohydrates have been reported in literature. What’s more, the functionalization is always via covalent linkages. Fluorous interaction is a new kind of noncovalent interaction which is defined as the affinity between two perfluorinated hydrocarbon chains. And this kind of interaction gradually attracts more attention since1990’s. Now it is widely used in biodetection, fluorous solid phase extraction, homogeneous catalysis, tagged synthesis etc. Recently it has been successfully used in carbohydrate microarray, which greatly reduces the non-specific adsorption as a result of the superhydrophobicity of the fluorous tag. As far as we know, research using fluorous interaction to detect the specific interaction of biomolecules in water has not been reported. The content of this thesis is as follows:1. Self-assembly of gold nanoparticles stabilized by fluorous ligand.The special properties of fluorous chemicals such as superhydrophobic, low surface tension induce different self-assembly behavior compared to their hydrocarbon counterparts, which inspires some research attention. However there are few reports about the preparation and self-assembly of gold nanoparticles stabilized by fluorous ligand. So we synthesize this kind of particles and use fluorous interaction to construct novel nanostructures. We use oleylamine as a weak reducing reagent, and subsequently use a mercapto fluorous ligand (1H,1H,2H,2H-Perfluoro-1-decanethiol) to stabilize the gold nanoparticles. And then we found that the fluorinated gold nanoparticles can self assemble into nanorod structures. And the nanoparticles inside the rods are arranged parallel. The distance between nanoparticles is about3nm. Further study shows that this self-assembly behavior is related to the fluorous ligand. And the fluorous content influences the morphology of the rod.2. Studies of the reversible aggregations of self-assembled fluorinated gold nanoparticles functionalized by sugar.Fluorous interaction has been successfully used in many research fields, especially in carbohydrate microarray for detection of carbohydrate-protein interactions. However fluorous interaction as a self-assembly driving force to construct hybrid nanostructures in solution has been rarely reported. Based on this research background, the fluorous ligand stabilized gold nanoparticles (F-AuNP) and fluorinated carbohydrates (F-Mal) were synthesized. They formed self-assembled structures with various morphologies both in DMSO:H2O (v:v=7:3) and water via different preparation methods. And the self-assembled structure can further aggregate into larger particles mediated by carbohydrate-protein interactions, which can be dissociated after addition of competitive ligands.