| Chirality is one of the fundamental properties of the nature, the activity of chiralcompounds is often different. Currently, chromatography is one of the most importantchiral analysis technology, the chromatography is limited in the application of chiralanalysis and test due to lack of optical rotation detector. This paper analyzes opticalrotation detection system from theory and experiment, and makes adjustment anddebugging for optical detection system.Theoretical studies and practical tests show that choosing light source plays animportant role in optical rotatory detect. The experiment finally decides to selectsmall size, low power, cheap LED as the light source of optical rotation detectthrough comparing several common light sources including laser, incandescent,incandescent combination filters and LED light source. In addition, using circledWZX-1optical spectrometer ensures only monochromatic light is passing, whichlight source replaced by a broad spectrum incandescent and remove the originalequipment filters, and install monochrome filters before light source and incident lensso as to verify optical rotatory dispersion effect.We has mde a prototype of optical rotation detector and debugged opticalrotation detector. The experiment accurately measure the variation of light intensityand polarization angle through light passing different glass tubes and the solutionsusing optical rotation detector. We find optical rotation detector can measurenon-chiral substance and right-left chiral substances through detecting water, glucosesolutions and L tyrosine measure, also, we can obtain the size of the optical ratationby measuring optical ratation of chiral substances. The optical rotation detector hasachieved the desired requirements, which can be used for fast measuring chiralsubstances. Further, we need to improve the direction by analyzing optical rotationdetector, and make foundations for next work. |