The Impact Of Epigenetic Modifier Saha On The BIO Activities Of Secondary Metabolites Of Two Symbiotic Fungi From Arctiumlappa

Chemical pesticides have caused serious environmental problems, thus increasing the demand for the relatively safe microbial pesticides. To obtain the biological activity pesticides, this paper carried out the two parts of work:Firstly, optimization of fermentation conditions of endophytic fungus NY-1.Small scale fermentation was carried in three fermentation medium, with different concentrations of epigenetic modifiers SAHA were added in. We choose to add 0.2 μmol/L of SAHA in PDB medium for large-scale fermentation. According to different polarity, the fermentation products were roughly divided into 12 sections, and activity tracking indicated that components E, F, G preferably had insecticidal activity. Particularly E, of which the median lethal ratio LD50 values as low as 11.37 μg/mL, is 2.89 times that of common chemical pesticides DDV. Components E, H showed medium antibacterial activity against Sa (Staphylococcus aureus), and component B showed 25% antibacterial activity against Ec (E. coli).Separation, purification and activity tracking were taken combined with various column chromatography, compounds B4B, B4D and E53 were purified. The LD50 value of B4B against Artemia is 126.76 μg/mL. Besides, the chemical composition of petroleum ether extract after activation were analysed by GC/MS.Secondly, the study of activation of endophytic fungus NY-3 from burdock by the chemical epigenetic modifiers (vorinostat).To activate the fungus NY-3 from burdock, the chemical epigenetic modifier SAHA were experimented at different concentrations during small-scale. With 100 μg/mL of fermentation broth before and after activation, insecticidal activity was found to be best. When activated by 20 μM of SAHA, lethality rate of NY-3 extract preferably reached 50.40%. Crude extracts of NY-3 before and after activation showed better bioactivities against common plant pathogens.