Preparation And Biocompatibility Of Phosphorylated Silica Nanoparticles

With the rapid development of nanotechnology, nanoparticles with special functions have been successfully produced. While the size of the nanoparticles greatly determined if they were applied in the field of chemistry and biology. As one of the most important members of inorganic nanoparticles, silica nanoparticles were widely used in biomarkers, DNA detection,gene carrier, medicine transport and so on. Due to extreme specific surface area and higher surface energy, silica nanoparticles are easy to reunite which inhibits their nanometer effect.In this paper, phosphonate-terminated silica nanoparticles (SiO2-P NPs) were obtained using water-in-oil (W/O) micro-emulsion. They were characterized with transmission electron microscopy (TEM), energy dispersive spectrometer (EDS), zeta potential ((?)) and Nuclear magnetic resonance (NMR). From the results above all, we got to know the morphology and structure of SiO2-P NPs.In order to explore the biocompatibility of SiO2-P NPs, UV-vis absorption spectra (UV-vis), fluorescence microscope (FL), circular dichroism (CD) and electrochemical methods were chosen to investigate the interaction between SiO2-P NPs and hemoglobin (Hb). Whole-cell patch clamp technique and cyclic voltammetry were used to research the cytocompatibility of SiO2-P NPs to the HEK293cell. Meanwhile, we recorded activated partial thromboplastin time (APTT), and prothrombin time (PT) and thrombin time (TT) of rabbit blood treated with SiO2-P NPs. According to the results, SiO2-P NPs could be better dispersed in water than SiO2NPs. Moreover, the surface modification on silica nanoparticles with phosphonate improved the biocompatibility of silica.All the results provided theoretical and experimental basis for the application in biomedical field of SiO2-P NPs.