Study On Chemical Composition And Antioxidant Activity Of Glycoprotein Acquired From Mucus Of Two Kinds Of Jellyfish

Jellyfish slime is a by-product during the jellyfish processing. The study on jellyfish slime could help to improve the production profit and reduce environmental pollution. The present work mainly focused on the basic chemical composition of slime from two jellyfish,Rhopilema asamushi and Stomopholus nomnrai. This study aimed to analyze the jelly mucus and promote its application in food, cosmetic and pharmaceutical fields.After pretreatment, two kinds of glycoprotein were obtained from these two jellyfish slime.Moisture content is analyzed by freeze-drying, total sugar content is determined by phenol-sulfate acid method, and the protein content is determined by coomassie brilliant blue method. Monosaccharide and amino acid compositions were analyzed by high performance liquid chromatography(HPLC). The molecular weights of the samples were analyzed by SDS-PAGE. Antioxidant activity of scavenging DPPH free radical and hydroxyl radical were determined by electron spin resonance(ESR).The mucus of R. asamushi contained moisture of 94.85%, total sugar of 3.57%, and protein of 33.54%. While the mucus of S. nomnrai contained moisture of 96.82%, total sugar of3.69%, and protein of 31.25%. The mucus of R. asamushi was composed of 7monosaccharides, namely glucosamine, mannose, aminogalactose, ribose, glucose, galactose and xylose, and 15 kinds of amino acids except Met,Cys,Ile and Trp. The mucus of S.nomnrai was composed of glucosamine, mannose, aminogalactose, ribose, glucose, xylose as well as 14 kinds of amino acids except Met,Cys,Ile,Trp and His. The mucus molecular weight of R. asamushi and S. nomnrai are both 97.2kDa. The half maximal inhibitory concentration of DPPH free radical and hydroxyl radical of R. asamushi were 17.51 mg/mL and 139.70 mg/mL, respectively, and those of S. nomnrai were 24.15 mg/mL and 150.19mg/mL, respectively.