Extraction, Purification, Stability, Antioxidation And Structural Identification Of Flavonoids From Prunus Persica Flos

As a kind of medicinal and edible plants with abundant resources, Prunus persica Flos has beauty and laxative activities. Flavonoid is one of the physiologically active ingredients in Prunus persica Flos. Here, the extraction, purification and stability, antioxidation activity of flavonoids from Prunus persica Flos were studied and their structures were preliminarily identified. The results as follows:1、The effect of different factors on extraction process of flavonoids from Prunus persica Flos was studied. The optimum extraction condition of flavonoids extraction is described as following: extraction temperature 50°C, ethanol concentration 70%, solid-liquid ratio 1:20, extraction time 90 min. Under the optimized conditions, the extraction ratio of flavonoids of peach blossom was 4.66%, the extraction rate could reach 5.62% after extracting twice.2、Between the six resins of D101, HPD722, DM130, LK17, HPD100 and polyamide, polyamide resin was the best purification resin. Under static conditions, the adsorption and desorption was fast, adsorption equilibrium time was 30 min, desorption equilibrium time is 40 min. The optimum temperature of adsorption and desorption were 20°C、30°C. Optimum conditions of dynamic purification were described as following: the concentration of sample was 3.6451 mg/mL, sample flow rate was 1.5 mL/min, eluent flow rate was 1 mL/min, the concentration of ethanol was 70%. After purifying three times, the purity of flavonoids was increased to 44.8%.3、By research on the stability of flavonoids from Prunus persica Flos, it showed as follows: the light had a certain influence on the stability of flavonoids of peach blossom; high temperature had great influence on the stability, flavonoids from Prunus persica Flos was relatively stable when temperature below 70°C、pH in the range of 3-10;flavonoids of peach blossom could easily be oxidated by H2O2. when in Zn2+、Cu2+、Cr3+metal ions solution, flavonoids was not stable. The tartaric acid, citric acid, glucose, sucrose had little effect on its stability.The results of antioxidant activity showed that, in the concentration of 25 μg/mL-250 μg/mL, the purified flavonoids had strong antioxidant activity, and compared to it before purification, the antioxidant activity of purified flavonoids was significantly enhanced. Among them, at the same concentration purified flavonoids reducing power weaker than the Vc, but higher than before purification flavonoids; Purified flavonoids on DPPH IC50 was 13.468 μg/mL; for ABTS + IC50 was 52.160 μg/mL; for ·OH the IC50 was 196.975 μg/mL.4、By HPLC-MS technology, the preliminary identification of purification flavonoids from Prunus persica Flos contains:kaempferol-3-O-glucosideor kaempferol-7-O-glucoside, kaempferol-3-O-rutinoside, kaempferol, quercetin3-xyloside, naringenin. The monomer which was further separated by silica gel column chromatography was identified as naringenin.