Study On Synthesis, Properties And Biological Application Of Fluorescence Detection Sensor Based On Important Yin And Cation

Because of the specificity, high sensitivity, simplicity of implementation and fast response time, fluorescent sensors offer applied in analytical chemistry, medical, environment and biochemistry. Fluorescent probes also become a new research hotspot in recent years.A new coumarin derivative1was examined for the sensing Pi, the results indicated that1is a new reactive turn-on fluorescent probe with high selectivity for Pi over other anions at pH7.4in aqueous media (DMSO/HEPES, v/v=9:1). Upon addition of Pi, it showed that Pi promotes a distinct color change of the probe from colorless to light blue, and a remarkable enhancement of up to780-fold in fluorescence intensity was observed. Compound1was successfully applied in living cells and nematodes to the fluorescence imaging and the operation of the enzyme. The Hela cells and nematodes were treated with ATP at first, then treated with ATP hydrolysis enzyme, then obvious fluorescence changes we can see. The significant fluorescence intensity was also observed as the Sf9cells loaded virus Inx2and Inx3. It was showing that1is a selective colorimetric and fluorescent chemosensor for Pi and that it can be employed to detect this important anion in vivo in HeLa cells and C. elegans and used to elucidate the mechanism of Inx3promoted apoptosis of hemichannel-closed Sf9cells.For the excellent selective sensing of Fe3+in aqueous media (EtOH/H2O, v/v=6:4), a new rhodamine-based fluorescent probe2was designed and synthesized. It showed that, Fe3+lead to a distinct color change of the probe from colorless to pink and along with strong green fluorescence intensity. However, we could not find the fluorescence changes in the presence of other cations except Hg2+. Compound2was successfully applied in living cells and nematodes. In BEAS-2B and Hela cells,2-Fe3+agent exhibited different fluorescence emission colors in the cell nucleus and in the cytoplasm. With titration of Fe+in c. elegans, we can see the fluorescence intensity change with different concentrations of Fe3+. In addition, compound2had light poisonous to HaCaT cells. This indicate that2is a highly selective and specificity sensor for Fe3+.