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Synthesis And Application Of Ion Fluorescence Probe Based On Common Fluorescent Dyes

Posted on:2018-08-23Degree:MasterType:Thesis
Country:ChinaCandidate:D D YeFull Text:PDF
GTID:2321330533959036Subject:Chemical Engineering
Abstract/Summary:PDF Full Text Request
Environmental pollution has always been a hot topic in today's society,environmental pollution testing has also aroused the concern of scientists,and has become an important area of environmental science research.Some of the metal ions and anionic excess will cause the ecological environment pollution,and cause harm to the human body,causing disease.Therefore,it is meaningful to study qualitative and quantitative methods for the detection of metal ions and anions.Fluorescence sensor technology in recent years has been rapid development,because the fluorescence sensor detection with good selectivity,high sensitivity,easy and easy operation of equipment,can be observed immediately,Fluorescence probe technology can be used in the ecological environment of ion detection and bioactive cell imaging technology,the practical application plays an important role.?1?A new type of fluorescence colorimetric probe was designed based on the principle that the metal ion could induce the open loop of the amide spiro ring in the rhodamine dye to cause the color and fluorescence to change significantly.The response characteristics of probe 1 to a series of metal cations were investigated.It was found that the probe showed only the color change and fluorescence enhancement,good selectivity,low detection limit and reversible reliability of Fe3 + and Cu2+ ions.The mechanism of selective response of Fe3+ and Cu2+ ions to probe 1 was discussed by using UV spectroscopy,infrared spectroscopy and density functional theory.The probe 1 was successfully tested for Fe3+ and Cu2+ ions in environmental samples by a simple test strip test.?2?Based on the strong affinity of the unique lactone structure and sulfur atom of rhodamine dyes,a novel Fe3+ ion fluorescence probe was designed and synthesized.The probe 2 can exhibit only the color change and fluorescence enhancement,high sensitivity and low detection limit response to Fe3+ ions in pure water environment.In the same way,the response mechanism of high selectivity of Fe3+ ions was discussed by UV fluorescence titration spectroscopy.The probe 2 was successfully used for the detection of Fe3+ions in living cells,indicating that the probe has potential application value in chemical sensing and biomimetic field.?3?Based on the principle of hydroxyl coumarin dye can easily use the hydroxyl site to control the fluorescence "open-light" and SO32-ion can induce acetylacetonyl groups to remove hydroxyl groups,designed for the novel synthesis of selective detection of SO32-anion fluorescence UV ratio probe 3.The spectral properties of the SO32-fluorescence UV ratio probe were investigated.It was found that the probe 3 exhibited a fluorescence enhancement,a linear increase in the UV ratio signal,a high sensitivity,and a rapid detection time to SO32-in the pure water environment.Probe 3 was successfully used for the detection of SO32-anions in living cells.The probe provides a platform for developing more fluorescent UV ratio probes.?4?Based on the coumarin fluorescence group design and synthesis of fluorescent probe 4,selective detection of ClO-anion.ClO-anion reacts with the amide on probe 4 to hydrolyze the amide to produce 6-carboxy coumarin to produce blue fluorescence.The spectral properties of the ClO-fluorescent probe were investigated.It was found that the probe 4 showed excellent spectral properties such as linear enhancement,high sensitivity and short response time for the ClO-anion in pure water.The response mechanism of probe 4 and ClO-anion was confirmed by detecting the ESI-MS spectrum of solution system after detection of probe 4 with ClO-anion.The probe 4 was successfully applied to fluorescence imaging experiments in living cells.
Keywords/Search Tags:Fluorescent probe, Rhodamine B derivative, Coumarin derivative, Biological cell imaging
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