Fluorescent Probes For Mercury Ion And Nitrite Ion Based On Benzanthrone

Mercury ion and nitrite ion can enter the human body through food and water and then cause physiological toxicity and great harm to human health. Thus, it is very important to detect them in the field of life science and environmental fields. Fluorescence assay has been widely used as a powerful technique for Hg²⁺ and NO^2- ions detections because of its ease of fabrication, low cost, high selectivity and good sensitivity.Benzanthrone has been used as fluorescent dyes because of its rigid planar biphenyl structure. In this thesis, a mercury ion fluorescent probe for imaging cells and a nitrite ion fluorescent probe for testing nitrite ion were synthesized based on benzanthrone.The method of fluorescent analysis and the design principle of fluorescent molecular probes were introduced herein. The research progresses of fluorescent probes for mercury ion and nitrite ion and the applications in biological and environmental fields were reviewed briefly. The research progress of fluorescent materials based on benzanthrone was described in detail.A thiolized benzanthrone（Probe I） was synthesized by the substitution and thionation of benzanthrone with Lawesson’s reagent and further characterized. A method of fluorescent analysis of detecting Hg²⁺ ion was established depending on the increase of fluorescent intensity after the reaction between Hg²⁺ ion and Probe I. Range of linearity for the concentration of Hg²⁺ ions was 0.05¹.0μmol/L. The limit of detection（LOD） was 0.19nmol/L. Furthermore, Probe I was successfully used in fluorescent imaging assay for detecting Hg²⁺ ion in the cells.A fluorescence reagent 3-ABA was synthesized and its fluorescent intensity increased after the reaction with NO^2- ion in the acid medium. The enhancement of fluorescent intensity indicated a linear relationship to the concentration of NO^2- ion in the range of 0.01⁰.6μmol/L when the emission wavelength was 413 nm and excitation wavelength was 566 nm. A method of fluorescent analysis of detecting trace NO^2- ion was established and possessed high selectivity and good sensitivity with the limit of detection（LOD） of 0.23nmol/L. This fluorescent analysis was successfully used to detect NO^2- ion in the water samples including the water of the Donghu Lake, rainwater and tap water. The recovery in the detection of NO^2- ion in water sample was between 93.0% and 102.0%.