Study On Encapsulation And Stability Of Nature Active Products

Nature active products (Astaxanthin and Sulforaphene), with many brilliant properties including antitumor, anti-cancer and antioxidants, offer a high innovative potential in pharmaceutical and food applications. However, it is easy for nature products to isomerizate and degradate under the condition of heating, acid, alkali, and oxidation. In order to improve the stability and bioavailability of nature products, microencapsulation technologies have been employed in this paper.Astaxanthin solid lipid nanoparticles (ASTA-SLNs) were investigated in section 1. The stability and release behavior of ASTA-SLNs were explored on the basis of the optimized preparation conditions. The physical properties (mean particle size, zeta potential and polydispersity index) of ASTA-SLNs were measured by dynamic light scattering technique with Nano Zetasizer. The results indicated that high pressure homogenization (HPH) was more suitable to prepare ASTA-SLNs. ASTA-SLNs with 1 wt% SA content had good physical stability, with mean particle size (170.3±12.7 nm), zeta potential (-31.5±3.1 mV) and PDI (0.19 ± 0.041). In addition, comparative with free ASTA, the stability of ASTA in SLNs was greatly enhanced. The release experiments of ASTA-SLNs revealed that SLNs could provide prolonged release of ASTA in simulated gastric and intestinal juices.Food-grade sulforaphene organogel was studied in section 2. The food-grade organogel was constructed and characterized. The analytical data of FTIR, XRD and DSC demonstrated that sulforaphene had a week influence on the the structure of food-grade organogel. The encapsulation efficiency (EE) could up to be maximum, when the mass ratio of soybean oil and stearic acid was 4:1. The organogelator stearic acid formed crystal structures in the cooling progress, which package sulforaphene into the three-dimensional frame. This viewpoint could be verified with the following stability and release experiments. By Contrast with free SFN, the chemical stability of sulforaphene was improved through food-grade organogel. The accumulative release rate of SFN loaded in SLNs could up to 83.85% at 14 h in vitro. This indicated that SLNs had a controlled action on the release behavior of SFN.