Study Of Effect Of Organic Amine On Calcium Carbonate Biomineralization

Calcium carbonate (CaCO3) is one of the most abundant minerals found in the world. Additionally, CaCO3 have been widely studied owing to their significance in biomineralization processes producing unique organic/inorganic material, biological activities of protein-adhesive properties, cell compatibility, hard tissue compatibility, and an attractive combination of properties, including opacity, high hardness, density, and low cost. All these characters attracted physicists, chemists and materials scientists to research on it. In this thesis, study of the effect of the cationic polymers and monomers as soluble and insoluble additives on the calcium carbonate mineralization, various series of calcium carbonate crystals with specific morphologies were produced. At the same time, in vitro calcium carbonate mineralization, the two different systems chitosan as cast films and freeze-dried scaffolds were studied, trying to figure out the impact of different systems under the organic amine together.By changing the experimental conditions, a series of special morphology of calcium carbonate crystals were obtained, the formation mechanism was also studied and discussed. The detailed results are summarized in the following:1. Using ammonium carbonate diffusion method, polymethyl acrylate, dimethylaminoethyl (PDMEAMA), methacrylic acid-dimethylaminoethyl methacrylate (DMEAMAM), N, N-dimethylethylenediamine (DMEDA) and N, N-dimethyl amine (MDEA) with different cationic polar groups, respectively, were used as calcium carbonate additives. Experimental results showed that in the presence of PDMEAMA and DMEAMAM step-by-step cube calcite were synthesized; in the present of DMEDA and MDEA, with the increase of the concentration the cuboid-shaped crystals gradually transformed into irregular cube-shaped. PDMEAMA acted some inhibitory effect on crystal growth.2. The impact of the co-effect of chitosan membrane and organic amine monomers with different electronic effects on calcium carbonate crystal morphology was examined. In different concentrations, since the molecular chain with different functional groups, the morphology of calcium carbonate changed dramatically. In the DMEAMA system, the promoting effect on crystal growth was found.3. The impact of the co-effect of chitosan freeze-dried scaffolds and organic amine monomers with different electronic effects on calcium carbonate crystal morphology was also examined. When the chitosan matrix was changed into freeze-dried scaffolds, at the same concentration of the additive system, the morphology of CaCO3 was changed significantly. At the same time, the concentration was also the key to modify the mineralization of CaCO3.