High Throughput Screening Of Photocatalytic Reactions Based On Fluorescence Imaging

In recent years, the industrial manufacturing industry, electricity industry, dyeing industry, and pharmaceutical industry have been developed rapidly. The development brings some benefits to our national economy, but also brings a series of environmental pollution problems. Air pollution, noise pollution and water pollution are becoming more and more serious. The reports emerge one after another, especially the water pollution. In the degradation of various kinds of wastewater, catalytic wet oxidation technology (CWAO) is a very popular technology. The technology is developed in the 80’s. It has been an advanced oxidation technologies of refractory wastewater to specially treat high concentration, high toxicity and high pollution. This technology focus on the multiphase wet catalytic reaction, but the development of high-performance solid catalyst is the key to this technology in wet catalytic reaction. It is reported that many researchers use one or more active metal or modified active metal to study degradation effect of wastewater. But this approach has a series of problems, such as high cost, the complicated process, or introducing other pollution in the process of wastewater treatment. There are a lot of metal elements in nature, and the chemical properties or shape or the treatment effect of wastewater is different about different metal elements. This paper is to establish a high-throughput screening method. This method focused on using different metal ratios to degrade wastewater and finally selected the optimal ratio of metal. This method greatly shortened the test cycle, reduced the manual labor, and improved the efficiency of the experiment.This article applied biological high-throughput screening technology to the screening of metal catalysts, and created a new method. In the process of screening catalysts, thousands of different metal ratios were filtered at the same time, and finally the optimal ratio of catalysts was selected. So this method improved the efficiency of the experiment. This experiment includes the following parts:the preparation of reaction chips (the preparation of hydrophobic coating), the selection of detection method, the development and operation of reactor (the design of spraying device and homemade airtight reactor), the operation of the simulated experiment (the degradation of the wastewater). Detailed experimental results are as follows:(1) The preparation of reaction chip. The process mainly includes the selection of substrate, the design of the grid size (designed a grid map of 500 * 500 um as the mask board), the coating of photosensitive gel, the adhesion of hydrophobic grid, the preparation and loading of nano-MoS2.(2) The selection of detection method. The experiment used fluorescence method for testing the result of the reaction. Fluorescence method is not only accurate and simple, but also testing the small substrate concentration. It can satisfy the requirement of high throughput screening. The experiment is operated through the metal sulfide on the degradation of fluorescein sodium solution. After the reaction, we can observe the change of fluorescent. If the fluorescent of reaction liquid became weak, it proves the catalyst has a fine activity.(3) The construct of reaction generator. The spraying device and homemade airtight reactor were mainly designed in this part. The application of the spray device can make the reaction solution into small droplets which fall on a chip. (Let small droplets uniformly fall into the grid).The homemade airtight reactor can be very good to prevent the evaporation of reaction solution, at the same time reduce the reaction space.(4) The operation and analysis of the simulated experiment. In the simulation experiments, we used the prepared molybdenum disulfide and metal sulfide composites to degrade fluorescein sodium. The dim spots of photograph were observed through the fluorescent of fluorescein sodium solution was reduced. And optimal ratio of the catalyst was selected. To prove the accuracy of the results, we made the relatively macroscopic experiment. Finally we got the catalysts we screened are really effective, and it is also proved that the method based on high-throughput screening technology of fluorescence imaging is feasible.