Preparation And Properties Of The Fluorescent Probes For Detecting Metal Ions Based On 1,10-phenanthroline

Metal ion exists in the natural environment and the cells of an organism widely. The amount of metal ion can affect the survival of organisms directly in the natural environment. In biology, the metal ion is also involved in a necessary component of important parts of organic life. Many enzymatic reactions are carried out with the needs of a certain metal ions, which can make the reactions goes smoothly. Therefore, it is of great significance to detect the content of metal ions fast, accurately and selectively.Five compounds of small molecules and conjugated polymer fluorescent probes have been synthesized by the reaction of Wittig and Schiff. The reaction is used the material 1,10-phenanthroline-5,6-dione oxidized by 1,10-phenanthroline with phosphorus salt Ye Lide and amino-compound respectively. Moreover, the five fluorescent compounds have been characterized by the spectra of 1H NMR, IR, elemental analysis and viscosity.The properties of five fluorescent probes to detect metal ions were measured by UV-vision absorption and fluorescent spectra. It includes the solvation effects in different salvations of the five fluorescent compounds, the selectivity and sensitivity of them to different metal ions. One of the conjugated polymer fluorescent probe compound has been coordinated with the element Eu in order to improve the intensity of the probe. In addition, the linear response range and detection limit of the conjugated polymer fluorescent probe have been calculated and the values of them were listed as fllows:0-0.6×10-5mol/L,2.47×1-4μmol/L for conjugated polymer fluorescent probe Ⅰ to Zn2+; 0-10×10-5mol/L,2.72μmol/L for conjugated polymer fluorescent probe Ⅱ to Fe3+; 0.8-10×10-5mol/L,0.12μmol/L for conjugated polymer fluorescent probe Ⅲ to Ag+; 0-1 ×10-5mol/L,9.3×10-3μmol/L for conjugated polymer fluorescent probe Ⅳ to Ag+; 0-0.6×10-5mol/L,2.59×10-3μmol/L for conjugated polymer fluorescent probe IV to Ag+, respectively.The experimental test results above all show that the five fluorescent probes own the good qualities of detecting metal ions correlative selectively and sensitively. They should be used to detect and distinguish metal ions in the natural environment and biological organisms, which is of great value in the future.