Study And Application On The Analysis Method Of Pesticides Residue In Three Edible Forest Foods

Edible forest product is a important food resources. Many forest products such as mushrooms, bamboo shoots and chestnut with its rich nutritional value, taste delicious, are more and more popular among domestic and foreign consumers. With development of forest foods industry, pesticides and fertilizer were inevitably used in the production process. Pesticide residue in food has become an important factor affecting the quality of forest products. Therefore, the establishment the method of determination pesticide residues in edible forest foods is very necessary. Using of new sample pre-treatment technology and advanced chromatography-mass spectrometry technology, the method of rapid detection of pesticide residues in bamboo shoots, mushrooms and chestnuts, was developed. It is important to guarantee the quality and safety of forest foods, for breaking international trade technical barriers and expanse of exports. The main results of this study were summarized as follows:(1) Mushroom samples was extracted with acetonitrile and cleaned up using dispersive solid phase extraction (DSPE) with primary secondary amine (PSA) and graphitized carbon black (GCB) sorbent. Then the target pesticides were determined and confirmed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) using external standard method. The recoveries of 14 carbamate pesticides in spiked mushroom ranged from 70.0% to 104.5% with the relative standard deviation (RSD) ranged from 3.2% to 9.1%. The method was simple, fast and sensitive. It also demonstrated that this analysis method was suitable for simultaneous determination of 14carbamate pesticides in edible forest food.(2) Chestnut were extracted with acetonitrile using microwave-assisted extraction technique (MAE) and cleaned up using dispersive solid phase extraction (DSPE). After cleaned using online gel permeation chromatography (GPC), the target 20 organophosphorus pesticides were rapidly detected by gas chromatography-tandem mass spectrometry (GC-MS/MS).The results showed that the average recoveries of 20 target pesticides were from 71.5% to 104.0% with the relative standard deviation ranged from 3.6% to 9.4%.Using several new ample processing technologies, interference from food matrix was effectively eliminated to improve the sensitivity of the method. It provided technical reference for the quality and export of the chestnut.(3) According to pesticides commonly used to pest control for bamboo shoots and environmental pollutants,26 organochlorine pesticides were studied as the target compound. The sample matrix solid-phase dispersion (MSPD) technique for sample pretreatment processing was simpler, faster, easier, less cost with higher reproducibility and recoveries than traditional liquid-liquid extraction. A gas chromatography-tandem mass spectrometry (GC-MS/MS) method was developed for simultaneous determination of 26 organochlorine pesticide residues in bamboo shoots.The average recoveries of 26 pesticides were varied from 81.1% to 108.9%, with relative standard deviations (RSD) between 3.3% and 9.5%. It indicated that the method had good accuracy and precision, which can meet the pesticides testing requirements.