Application Of Research On The Acid Mine Drainage Treatment Technology By Sulfate Reducing Bacteria

It’s a very potential technology that apply Sulfate reducing bacteria method to deal with acid mine drainage at present. The sludge In this article come from the Huhhot Zhanggaiying backflow of sewage treatment plant of the second pond sludge, Through the simulation of acid mine drainage domestication, we got containing sulfate reducing bacteria (SRB) in anaerobic sludge, and we studied SRB for sulfate reduction properties under different pH conditions in the process of domestication, set The Cu2+, Zn2+,Ni2+ and Cr6+ these four kinds of heavy metal ions as the experimental factors, investigated the removal efficiency of SRB and the recovery performance under different restoration measure after the sludge "poisoning". Besides, we analyzed the mechanism of SRB to remove heavy metal ions, form and the functional bacteria flora. Get the following results in this paper.(1) The system pH of return sludge stable at about 7.83 after 19 days of the early domestication, The average removal rate of COD was 50%, the sulfate radical removal rate is 64% on average.Under different water pH conditions (pH 4,5,6)in late domestication, When the water pH=5, the treatment effect is best, the sulfate radical has the highest removal rate.(2) In the different heavy metal ions water loading conditions, (3.16mg/(L-d)> 3.87mg/(L·d)、5.46mg/(L·d)), removing the heavy metal ion of SRB effect is different, the removal efficiency of SRB for Cu2+ and Zn2+ are better than Cr6+ and Ni2+.(3) Recovery measures were taken to the system, (like reducing the burden on the environment, Remove heavy metal ions etc), it takes 16 days to recover when water load is 3.87mg/(L·d), it takes 23 days to recover when water load is 5.46mg/(L·d). Hence, the method of the water load 3.87mg/(L·d)recover faster than the water load is 5.46mg/(L·d).(4) It turned out that by using XRD to analysize the sludge samples containing metal,Cu2+、Zn2+ andNi2+ removed mainly by sulfide precipitation method, the Cr6+ is being used by SRB deoxidizing into Cr3+ in directly and removed by sulfide precipitation, Zn2+ are removed in the form of Zn(OH)2.(5) We used scanning electron microscopy (sem) instruments to take pictures after processing the HRT=31h sludge samples, SRB like a rod and spherical,at the same time we can also observed metal sulfide precipitation. Applying PCR amplification into the selected nine segments of 16SrRNA, the results of No.19, No. 23 and No.24 fragment sequencing showed that desulfovibrio are part of SRB,which play an important role in the process of acid mine drainage.