Objective is to study Fe3+ catalyses ginsenoside transforming ginsenoside PPD to ginsenoside Rg3, Rk1 and Rg5. The best condition of the catalyzation by Fe3+ can be find. And purification and isolation were studied.The optimal fermented conditions for the main catalysate Rg3 were studied. The concentration of Fe3+ was 1050 mmol/L, the temperature was 50 ℃, the optimal reaction time was 10 hour, the optimum substrate concentration was 1.6%.AB-8 macroporous resin was eluted by 46% ethanol for seven times bed volume,66% ethanol for five times bed volume. Rg3, Rk1 and Rg5 were purified by silica gel column. Their yield were 14.10% and 17.10%.The optimal fermented conditions for the main catalysate Rg5, RK1 were studied. The concentration of Fe3+ was 1800 mmol/L, the temperature was 5 ℃, the optimal reaction time was 88 hour, the optimum substrate concentration was 0.1%. AB-8 macroporous resin was eluted by 46% ethanol for six times bed volume,66% ethanol for seven times bed volume. Rg3, Rk1 and Rg5 were purified by silica gel column. Their yield were 3.32% and 18.91%. |