Preparation Of Anticoagulant Hyperbranched Polymers And Their Properties

Hyperbranched polymers have attracted significant interests because of their unique architecture and high density of their functional groups. Herein, the aliphatic water-soluble hyperbranched polyester nanoparticles with sulfonic acid functional groups (HBPE-SO3 NPs) were synthesized and characterized. They are amphiphilic polymeric nanoparticles with hydrophobic hyperbranched polyester (HBPE) core and hydrophilic sulfonic acid terminal groups. The biocompatibility and anticoagulant effect of the HBPE-SO3 NPs were investigated using coagulation tests, hemolysis assay, morphological changes of red blood cells, complement and platelet activation detection, and cytotoxicity. The results comfirmed that the sulfonic acid terminal groups can substantially enhance the anticoagulant property of HBPE, and the HBPE-SO3 NPs have the potential to be used in nanomedicine due to their good bioproperties.To test and verify the above method had general applicability, the water-soluble hyperbranched polyester nanoparticles with carboxylic acid functional group (HBPE-CA NPs) are synthesized and characterized. They have amphiphilic structure that include HBPE core and hydrophilic carboxylated terminal groups. Biosafety assessment tests of the HBPE-CA NPs include coagulation times, hemolysis, complement activation, platelet activation and cytotoxicity are performed. The results show that the HBPE-CA NPs exhibit good hemocompatibility that strongly depend on the amphiphilic structure. Moreover, the results also indicate the non-cytotoxicity of the HBPE-CA NPs.To improve the hemocompatibility of glass, the surface of glass was modified by DMPA to build the hyperbranched polyester structure. We characterized the HBPE modified glass (G-DMPA) by fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, atomic force microscope and water contact angle. Anticoagulation assessment tests include platelet adhesion experiment and whole blood adhesion. The results indicate that the anticoagulant property of G-DMPA surface was enhance by HBPE structure.