The Preparation, Characterization And Bioelectrochemical Application Of Ionic Liquids Based Polysome

Direct electrochemistry of redox proteins has aroused great interest in biological and bioelectrochemistry fields. Study on direct electrochemistry of redox proteins is of great importance both for studying the intrinsic redox behaviors of proteins and fabricating new kinds of biosensors without addition of mediators. In this dissertation, electrodes are modified with ionic liquids based polysome to investigate the electrochemical and electrocatalytic properties of heme-enzymes. This research may open up new opportunities for designing novel third generation of biosensors. More details are summarized below:1. Ionic liquids based lipid and liposome were synthesized firstly. On the basis, ionic liquids based polysome was prepared via the radical polymerization of liposome, then improved to ionic liquids based polysome, that is ionic liquids based polysome happened ion exchange with chloroauric acid to prepare Au nanoparticles modified ionic liquids based polysome. The structure, surface properties and morphology of polysome and liposome were characterized by FT-IR, TEM and DLS. The experiment results demonstrated that ionic liquids based polysome exhibited excellent stability. Compared with liposome, the capsule composed of the same lipid, polysome could maintain the original structure and morphology under dry state. After improving to it, ionic liquids based polysome still has a good fixed action to Au nanoparticles.2. The as prepared ionic liquids based polysome and Au nanoparticles modified ionic liquids based polysome were firstly utilized as electrode material to form HRP/Polysome/PVA/GC and HRP/Polysome-Au/PVA/GC modified electrode together with horseradish peroxide (HRP). Since the polysome exhibited the properties of two elementary materials, such as the conductivity of ionic liquids and the biocompatibility of liposome, it can efficiently promote the electron transfer of the HRP. Based on that, the direct electron transfer between HRP and glassy carbon electrode could be well achieved. Such modified electrode also exhibited electrocatalytic performance toward NaN02 and H2O2. HRP/Polysome-Au/PVA/GC modified electrode exhibits more better than HRP/Polysome/PVA/GC modified electrode in electrochemical performance, meanwhile, achieve rapid detection of NaN02 and H2O2. The nanocomposite material which possess well biocompatibility exists potential applications in direct electrochemistry and signal transmission in the biological system.