Effect And Mechanism Of Inositol And D-Chiro-Inositol On Alcoholic Fatty Liver Rats

Wine culture has a long history in China.Today,people cannot leave drinking in social activities.Fatty liver induced by alcohol(AFL) and the development of severe liver disease have became major threaten to human health.Inositol is a kind of water-soluble natural substances which are easy to be absorbed.Inositol widely exists in organizations of various animals,natural plants and microbe. Myo-inositol(easily called inositol) and D-chiro-inositol are two isomers of inositol which can promote the metabolism of liver fat and lower the content of cholesterol in the blood. This experiment takes SD rats as animal model,researching the effect of inositol and D-chiro-inositol to AFL rats.Then explore the possible mechanism of inositol and D-chiro-inositol to AFL at the molecular level.Firstly,experimental animals were randomLy divided into 6 groups:blank control group,model group,high-dose inositol group(200mg/kg·BW·d),low-dose inositol group(100mg/kg·BW·d),high-dose D-chiro-inositol group(200mg/kg·BW·d),low-dose D-chiro-inositol group(100mg/kg·BW·d).We use the fraction of 40% alcohol to fill the rat’s stomach.We lavage the rats two times a day,two more than 6 hours apart.Different dose of Inositol and D-chiro-inositol were lavaged at the same time.Measuring body weight,visceral index,serum and liver biochemical indicators 60 days later.Do a liver pathological observation.Then we apply the technology of realtime-PCR to test the targeting genes(PPAR-γ, TNF-α, Adiponectin)which are concerned with AFLThe results showed that inositol can reduce rat liver index,Inositol and D-chiro-inositol both can lower serum TC and TG levels and inhibit the activity of serum AST. The two doses of inositol can remarkably reduce liver TC level(P<0.05),the high dose group can extremely remarkably lower liver TC level(P<0.01). The two doses of D-chiro-inositol can remarkably reduce liver TC level(P<0.05).The two doses of inositol and D-chiro-inositol both can extremely remarkably lower liver TG level(P<0.01).High-dose inositol group can elevate liver GSH-Px activity remarkably (P<0.05),reduce liver MDA concentration extremely remarkably(P<0.01);High dose of D-chiro-inositol can lift liver SOD activity extremely remarkably(P<0.01),and extremely remarkably lower MDA level(P<0.01). The liver biopsy indicates that inositol and D-chiro-inositol can relieve hepatic stestosis,prevent AFL to a certain degree. Inositol and D-chiro-inositol can reduce the content of liver lipid,inhibit alcoholic fatty liver.The effect of inositol and D-chiro-inositol is similar.The Realtime-PCR results showed that alcohol lavage can extremely remarkably increase the expression of TNF-α and PPAR-γ(P<0.01),and extremely remarkably lower the expression of Adiponectin receptor 2(P<0.01) which belongs to liver of rats.The four doses groups can extremely remarkably lower the expression of TNF-α mRNA (P<0.01).The two doses of inositol and the low dose of D-chiro-inositol can extremely remarkably lower the expression of PPAR-γ mRNA(P<0.01),the high dose of D-chiro-inositol can remarkably lower the expression of PPAR-y mRNA(P<0.05).The expression of Adiponectin receptor 2 mRNA of the four doses groups has no remarkable difference with the model group.The Realtime-PCR results indicates that the mechanism of inositol and D-chiro-inositol to AFL rats is concerned with inhibiting the expression level of TNF-αmRNA and PPAR-γ mRNA.