Study On The Application Of New Nanomaterials In Immunochromatographic Test Strip For The Determination Of Salbutamol

In this study, three kinds of labels:colloidal gold, colloidal carbon and nanogold-polyaniline-nanogold microspheres (GPGs) were studied and applied for immunochromatography test strip to determine Salbutamol(SAL) respectively. Conditions of the designed strips procedure were optimized.An immunochromatographic rapid test strip based on colloidal gold as label was developed for the determination of salbutamol. The detection limit of the SAL test strip with colloidal gold-labeled antibody jetted into the conjugate release pad was 2 μg L-1. The detection limit of the SAL test strip with colloidal gold-labeled antibody lyophilized on microplate was 1 μg L-1. Pork, beef, lamb and liver were used as real samples, and the detection limit of samples were 5μg L-1.A new type of nanomaterial named GPG was synthesized, and immuno-chromatographic rapid test strip based on this material has been developed. The detection limit of the test strip with GPG-labeled antibody jetted into the conjugate release pad was 5 μg L-1. The detection limit of the SAL test strip with GPG-labeled antibody lyophilized on microplate was 2 μg L-1. Pork, beef, lamb and liver were used as real samples, and the detection limit of samples were 10 μg L-1.The average particle size of SB4 carbon black was 50 nm, and immuno-chromatographic rapid test strip used colloidal carbon as a visual marker has been developed. Assemblage method with colloidal carbon-labeled antibody jetted into the conjugate release pad was infeasible. The detection limit of the test strip used two other assembly methods was all the same, which was all 2μg L-1. Pork, beef, lamb and liver were used as real samples, and the detection limit of samples were 10μg L-1.The sensitivity of test strips utilized three different kinds of nanomaterials respectively was nearly the same. For GPG and colloidal carbon, the differents between batches were small, and the stability of these two materials was much better than colloidal gold. The price of colloidal carbon was much lower than colloidal gold, what’s more, used colloidal carbon as a visualized label could save nearly 11 times antibody compared with colloidal gold. Used colloidal carbon as a label to develop an immunochromatographic rapid test strip can reduce the cost, and the productions easily scaled up.