Effect Of Stimulated Vibration And Impact On The Qualities Of Fuji Apples And Its Molecular Mechanism

Fruit production is so big in our country, but fresh fruit whether on-site storage, under-site storage, or sell everywhere, it’s usually from circulation field to be the product into the evolution process of the goods. So fresh fruits during the transportation and handling, no matter what kinds of transportation, packaging. vibration, shock, extrusion, collision is inevitable, it can damage the fruit tissue structure in different degrees, and produce various damage, such as skin damage, and because of the change of temperature and humidity, fruit tissue destruction, and the change of physiological characteristics such as the increasing of respiration intensity, at the same time it can lead to metabolic changes. Therefore, in the process of circulation, the loss of fruit caused by vibration, shock can’t be ignored. Circulation and packaging is an important stage of fruit storage and sales, it can reduce the fruit injury through research the change of the circulation of fruit quality and the analysis of mechanism. In the process of circulation and cushion packaging method and the damage resistance of fruit varieties provides a better theoretical basis.In this paper, during simulating transportation vibration and shock treatment, it has an effect on physiological and biochemical quality and ethylene biosynthesis and signal transduction related enzymes of the molecular mechanism of gene expression of Fuji apple. Main research results are as follows:1. Vibration has different degrees of effects on fruit physiological and biochemical indexes, two types of temperature under the condition of 20 ℃ make fruit best edible period, and the storage stage after the vibration and the late vibration of the fruits of various physiological activity indexes are the trend of first increased and then decreased, after vibration 9 h,12 d ethylene production, the ACC content and activity of ACS, the ACO activity of Fuji apple changes significantly, decreased significantly later; 4℃, the discharge of ethylene peak 44.4 uL/kg·h during the vibration 9 h; 20℃, the ethylene production is maximum 60.0 uL/kg·h during the vibration 9 h, vibration in the late storage to 12 d, generation of ethylene peak is 20.0 uL/kg·h, then decreased significantly.2. Ethylene biosynthesis pathway gene expression level is related to enzyme activity change trend, Fuji apple of vibration 6 h,8 d storage processing MdACS1, MdACO1, MdCTR1, MdETR1, MdERS1 gene expression level has reached the maximum, decreased significantly later. And ACS activity level change more significantly than ACO gene, MdACS1 gene were significantly in MdACO1(P<0.05), ethylene transduction via MdCTR1 in gene expression level change more significantly than MdETR1 and MdERS1(P<0.05).So, Fuji apple from mechanical damage, especially under the condition of vibration can produce different kinds of physiological changes, the organization for climacteric variant of Fuji apple. Vibration can lead to the increasing of ethylene release. Lower hardness can lead to fruit mature more rapidly, shorten the storage period, the cell walls of fruits organization can be destroyed, accelerate fruit rot, vibration destroy the balance of the fruit itself, but the fruit can resist the external environment to make their own nutrients supply level normally in the short term, physiological metabolism is slow, after that entering the aging.3. Compared with the vibration, impact has a significant effects on fruit, impact height of 30cm marble material for fruit ethylene bio-synthesis pathway and physiological activity index change more significantly, the impact of the more than 30 cm high fruit various physiological and biochemical decrease,30 cm of height to make the fruit can withstand the maximum threshold, and the fruits are the most activity, when the high impact is 30 cm, and the impact of the marble material material of ethylene release from 12.2 uL/kg·h to 39.2 uL/kg·h, after 30 cm, down to 26.0 uL/kg·h, change significantly (P<0.05). Fuji apple are the most activity, the increasing of ethylene release, and the increasing of ethylene biological pathways associated with ACS, the ACO activity.4. M7ACS1, MdACO1, MdCTRl, MdETR1, MdERS1 gene expression abundance is the most significant (P< 0.05) related to shock of Fuji apple, and the ACS activity level change more significantly than the ACO, MdACS1 gene change more significantly than MdACO1, in ethylene transduction via MdCTR1 gene expression level is more significantly than MdETRl and MdERS1 gene expression (P< 0.05).So, ACS and CTR play an important role in ethylene synthesis pathway of impact. On the other hand, Fuji apple breathing the most significant level after impacting, carbon dioxide release quantity is the largest, Fuji apple metabolism accelerate ripe and short the shelf life. For different material impact material, marble material is the most hardness, HDPE plastic is most soft, followed by corrugated cardboard and plastic foam, so the most hardness material impact on physiological and biochemical fruit and gene expression level have a huge effect, the fruit packaging materials should be used by plastic foam material.