Preparation And Performance Of Enzyme Induced Organic/Inorganic Hybrid Materials

Recently, for most of enzymes the enzymatic activity could be enhanced dramatically in enzyme–inorganic hybrid nanoflowers. Not only the enzymatic activity was enhanced, but the stability and reusing availability of enzymes was also improved. In addition, the production process of enzyme–inorganic hybrid nanoflowers is mild, convenient and ecofriendly, without involving high temperature or any organic solvents. A step change in enzyme engineering and biological catalysis will be taken by the found of enzyme–inorganic hybrid nanoflowers. Thus, several relative researches have been reported during the later years. The challenge is to explore appropriate substrate materials to deepen the underlying behavior and extend the working spectrum of the enzyme–inorganic hybrid nanoflowers. However, rarely researchers pay attention to other inorganic materials, new structures and strategies will certainly be novel.In this paper, the first three chapters mainly studied the nanostructure and enzyme activity change of composite materials between lysozyme, laccase, carbonic anhydrase and copper phosphate, graphene oxide, carbon nanotubes. The production process of composite materials is very mild and convenient, without special instruments or any organic solvents. The final preparation of composite materials have a certain rule of microstructure, belongs to a result of three-dimensional self-assembly. In terms of performance, compared with individual free lysozyme, GO,and even copper phosphate, GO/lysozyme/copper phosphate nanocomposites show excellent antibacterial activity, which is about ten-fold higher than that of the free enzyme. Composite materials of laccase showed high efficient enzyme immobilization and improved enzyme activity, kinetics tests proved that the stronger affinity for the substrate. In addition, the composite material of laccase showed a strong advantage for dye removal and better storage stability. Composite materials of carbonic anhydrase is no increase in enzyme activityl, but get a high degree of retention, the fixed amount of carbon dioxide is better also, and the thermal stabilityis also improved.Finally a piece of content of this article mainly focuses on carbonic anhydrase,carbonic anhydrase was studied with other metal phosphate, which could create nanoflowers. Experiments proved that carbonic anhydrase could form uniform nanoflowers with copper phosphate, octacalcium phosphate, manganese phosphate.The octacalcium phosphate has a biggest function on the promoting carbonic anhydrase enzyme activity, copper phosphate could load the largest enzyme, in addition, phosphate copper and octacalcium phosphate is better performed in the repeated use of carbon dioxide fixation.This study could provide a new method of three-dimensional self-assembled graphene oxide and carbon nanotube, which is expected to have many potential applications including catalysis science, antibacterial materials, and biological research.