The Study Of Composite Nanoparticles Of Polyelectrolyte-graphene-Au/Ag: Preparation And Application

To fabricate functionally integrated hybrid nanoparticles holds high importance in biomedical applications but is still a challenging task. In this study, we report multi-functional reduced graphene oxide(rGO)-nobel metal hybrid particles that present simultaneously the photothermal, surface-enhanced Raman spectroscopy(SERS) effect and biotherapy. The hybrid particles where spiky noble metal particles were wrapped within rGO polyelectrolyte layers were prepared by a facile and controllable method. rGO template modified using polyethylenimine(PEI) and poly(acrylic acid)(PAA) via layer-by-layer technology served as the reactive precursors, and the morphologies of the particles could be facilely controlled via controlling the number of bilayers around the rGO template. The hybrid particle presented low cytotoxicity. After loading doxorubicin hydrochloride, the particles effectively induced cell death, and photothermal treatment further decreased cell viability. We expect the reported method provides an effective strategy to prepare rGO-noble metal hybrid nanoparticles that find potential biomedical applications.The preparation of polyelectrolyte-graphene composite nanosheet was firstly studied. The structure of the polyelectrolyte-graphene film were characterized using FTIR, DLS, TEM, and AFM measurement. Secondly the prepared polyelectrolyte-graphene composite nanosheet was used as a reactive precursor to fabricate the noble metal-polyelectrolyte-rGO composite nanoparticles. The morphologies and structure of the hybrid particle were analyzed in detail by using UV-vis, FTIR, TEM and SEM measurement. The composite nanoparticle prepared using the optimized parameters presented superior photothermal effect and surface-enhanced Raman spectroscopy(SERS) effect, and a propore diameter of around 100 nm.Finally, the therapy effects of the drug-loaded composite nanoparticle towards Hela cells were studied. The composite particles presented low cytotoxicity before drug-loading, but effective kill Hela cells after loading DOX. IR irradiation further promote drug release and promote cell mortality. The results indicate the as-prepared composite nanoparticles hold high promise as biomedical materials.