Preparation And Evaluate Of A New Class Of Brush Chiral Stationary Phase

Most of the life activities are carried out in a chiral environment, chiral drugs affect different between enantiomers of different life activities, which prompted rival drug we must achieve between different pairs of enantiomers separation. Currently, high performance liquid chromatography in the separation of chiral drugs analysis plays an important role.This article will o respectively acetylsalicylic acid chloride, the reaction 3,5-hydroxy-nitrobenzoyl chloride with different positions of cellulose synthesis of novel cellulose derivatives [6-(2-acetoxy benzoate)-2, 3-(3,5-dinitrophenyl formate)]- cellulose(CLD-4). As a chiral recognition agent, silica gel as a carrier, γ-aminopropyl triethoxysilane(KH-550) and γ-(2,3- glycidoxypropyl) trimethoxysilane(KH- 560) for the silicone modifier, coating and bonding were used in two ways, synthesized two novel cellulose derivatives chiral Stationary phase CSP-1 and CSP-2, and determines the two fixed-phase synthesis process condition. By thermal gravimetric analysis, infrared analysis, means of elemental analysis and particle size analysis, and the purpose of the intermediate products prepared were characterized illustrates the synthesis of the desired product is successful.In order to investigate the synthesis and application performance stationary phases, firstly coated Stationary Phase CSP-1 solution of N- acetyl-DL- phenylglycine, DL-3- phenyl lactic acid, tioconazole, terbutaline, econazole other five enantiomers were split and examined the individual enantiomers more suitable mobile phase composition and the effect of mobile phase flow velocity on the effect of the split.Secondly, the use of Bonded Stationary Phase CSP-2 pair of rings Lunte Luo, tulobuterol, isoproterenol, promethazine, econazole, terfenadine, DL-2- benzene Gan ammonia alcohol, 2 fixed-phenyl-3-hydroxy acid, eight kinds of chiral separation of enantiomers split, not only to achieve a successful separation, and also compare the preparation of the forward reaction and the reverse reaction relative relevance enantiomer split effect and found a positive effect of the split-bonded stationary phase than the reverse.The study found, the bonding column on the choice of mobile phase and split effect, are better than coated column; species split chiral drugs of two different phases of the split range, but a certain complementarity.