Enrichment And Detection Of Beer Spoilage Bacteria

PCR (polymerase chain reaction) is a fast and convenient in DNA amplification me thod, always used for bacterial identification and other fields. This method is easy to operate, high sensitivity, short detection time, etc. This study will use in rapid detecti on of PCR beer bacteria pollution.8 strains were separated and purified from beer、wine、fermented liquid, used clas sical medium detection method, combined with fatty acid appraisal method as first st ep determined. Finally 8 strains were identified though PCR sequencing method, respe ctively are:Lactobacillus plantarum (JSB), Lactobacillus parabuchnei (White), Sta phylococcus saprophyticus (Ball), Lactobacillus brevis BSO 464 (47), Lactobacillus brevis (49), Lactobacillus farciminis (50), Lactobacillus brevis KB290 (58), Lacto bacillus brevis M8 (61)In addition, according to a lot of reference and test screening of bacteria spoilage, r esearch on optimization of bacteria pollution in the beer before PCR best conditions i n the process of the enrichment culture (including the best proportion of medium co mponents, table speed, pH, add growth factor), the test steps in the whole process of rapid test is crucial, but so far the detailed research reports are rare. In this paper, t hrough optimizing the traditional MRS culture medium to obtain the improved MRS c ulture medium, and factor of influence on bacteria growth process optimization, the re sponse surface analysis makes the test bacteria in 24 hours after the liquid culture in creases the amount of bacteria increased by 182%.Reference 41 strains common Lactobacilli HorA、HorC beer hop sequence, designed three pairs of specific primers, experiments show that these primers are specific to d etect the beer contaminated bacteria were isolated. With beer, while bacteria pollution of 16SrDNA combined with multiple PCR primers, specificity is greatly enhanced. Sen sitivity of single primer PCR is 5 cfu/mL.