The Effect Of Temperature On The β-glucosidase Activity In The Green-making Tea Leaves

The designer took 3 different kinds of tea leaves in progress as the subjects of the experiment, including Meizhan, Qilan, Jin guanyin. Under 3 different temperatures, including 17℃、22℃ and 27℃, two sets of test tea leaves in progress would be under observation. One set of test tea rested, the other were dealt with green making. After observing β-glucosidase activity and water content in 4 kinds of teas, the writter drawed a conclusion and summered up the rule of the change of β-glucosidase activity and water content in the process of green making. The result indicates that:In the process of green making, the change of water content of different temperature are presented as below:Meizhan:rose, fell at 17℃,22℃ and 27℃.Qilan:fell at 17℃ and 27℃; fell, rose and fell at 22℃.Jin guanyin:fell at 17℃; fell,rose,remain the same and fell at 22℃; fell, rose and fell at 27℃.At different temparature, the water content of the above 3 kinds of tea leaves in every procedure of the green-making progress are presented as below:A2B2<A2B1<A2B3; A2B3< A2B1 <A2B2; A2B1 <A2B2< A2B3In addition, the test for significance of difference of water content of tea leaves between test on standing and test in green making at 17℃, and at 22℃ and 27℃ would reach a significant level in some procedures, including Meizhan’s before third rocking, before forth rocking and after green making; Qilan’s before second rocking, before third rocking, before forth rocking and after green making; Jin guanyin’s before second rocking, before forth rocking and after green making. Besides, the test for significance of difference of water content of tea leaves at 22℃ and at 27 ℃ respectively had reached a significant level.In the process of green making, the change of β-glucosidase activity of different temperature are presented as below:Meizhan:rose, fell and rose at 17℃; fell and rose at 22 ℃; fell, rose, fell and rose at 27℃.Qilan:rose, fell and rose at 17℃ and 27℃; rose and fell at 22℃.Jin guanyin:rose, fell, rose and fell at 17℃ and 22℃; fell and rose at 27℃.At different temparature, P-glucosidase activity of the above 3 kinds of tea leaves in every procedure of the green-making progress are presented as below:A2B3< A2B2< A2B1; A2B1< A2B3< A2B2; A2B1< A2B2< A2B3Test for significant difference indicated that 3 above tea leaves in processing had existed a significance of difference in different procedure at different temperatures.At the same temperature, comparing with the set of test on standing, the average moisture content of Meizhan, Qilan and Jin guanyin in green making were less(except Jin guanyin was contrary at 22 ℃). At 17℃, comparing with the set of test on standing, β-glucosidase of Meizhan, Jin guanyin in green-making progress were less active, yet, Qilan was contrary. At 22 ℃, comparing with the set of test on standing, β-glucosidase of Meizhan, Qilan in green-making progress was less active, yet, Jin guanyin was contrary. At 27 ℃, comparing with the set of test on standing, β-glucosidase of Meizhan in green-making progress was less active, yet, Qilan and Jin guanyin were contrary.